Derivatization with 4-chloro-7-nitrobenzofurazan for liquid chromatographic determination of hydroxyproline in collagen hydrolysate.
1981
M Ahnoff, and
I Grundevik, and
A Arfwidsson, and
J Fonselius, and
B A Persson
UI
MeSH Term
Description
Entries
D009327
4-Chloro-7-nitrobenzofurazan
A benzofuran derivative used as a protein reagent since the terminal N-NBD-protein conjugate possesses interesting fluorescence and spectral properties. It has also been used as a covalent inhibitor of both beef heart mitochondrial ATPase and bacterial ATPase.
Chromatographic techniques in which the mobile phase is a liquid.
Liquid Chromatography
D003094
Collagen
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).