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Quantification of bile acids has been carried out by selected ion monitoring and by repetitive scanning. The latter method has been explored critically and found to be very suitable. A 24 h collection of feces was submitted to Soxhlet extraction. The extract was purified on Servachrom XAD-2, and separated into sulfated and non-sulfated fractions over DEAP-Sephadex-LH20. Conjugated and sulfated bile acids were enzymatically deconjugated and desulfated prior to permethylation. Deuterated bile acid derivatives were added as external standards and an aliquot was injected onto a capillary column of SE-52. The reconstructed chromatogram traces of specific ions were integrated to quantify known bile acids. Spectra of all metabolites were available for identification purposes simultaneously.
G A De Weerdt, and
R Beke, and
H Verdievel, and
F Barbier
September 1977,
Clinica chimica acta; international journal of clinical chemistry,
G A De Weerdt, and
R Beke, and
H Verdievel, and
F Barbier
July 1969,
Zeitschrift fur klinische Chemie und klinische Biochemie,
G A De Weerdt, and
R Beke, and
H Verdievel, and
F Barbier
July 1972,
Clinica chimica acta; international journal of clinical chemistry,
G A De Weerdt, and
R Beke, and
H Verdievel, and
F Barbier
November 1971,
Journal of lipid research,
G A De Weerdt, and
R Beke, and
H Verdievel, and
F Barbier
January 1981,
Laboratornoe delo,
G A De Weerdt, and
R Beke, and
H Verdievel, and
F Barbier
July 1974,
Zeitschrift fur Gastroenterologie,
G A De Weerdt, and
R Beke, and
H Verdievel, and
F Barbier
June 1963,
Analytical biochemistry,
G A De Weerdt, and
R Beke, and
H Verdievel, and
F Barbier
July 1978,
Analytical biochemistry,
G A De Weerdt, and
R Beke, and
H Verdievel, and
F Barbier
April 1980,
Journal of chromatography,
G A De Weerdt, and
R Beke, and
H Verdievel, and
F Barbier
May 1964,
The Biochemical journal,
