Biomaterial Database

The protective action of glycols against freezing damage of mouse and rat embryos. 1978

H Miyamoto, and T Ishibashi

Eight-cell mouse and rat embryos were frozen to -79 degrees C or -196 degrees C in the presence of ethylene, diethylene, triethylene, propylene or polyethylene glycol. Ethylene glycol was the most effective cryoprotectant for mouse and rat embryos and considerable protection against damage during freezing and thawing was also afforded by propylene glycol. The degree of protection given by the other glycols was relatively low. Mouse embryos survived freezing after only 0.1 min exposure to ethylene glycol at 0 degrees C but exposure for longer periods was necessary with the other glycols. Mouse embryos that survived freezing and thawing with a glycol as the protective agent were capable of developing to full-term fetuses.

UI	MeSH Term	Description	Entries
D011309	Preservation, Biological	The process of protecting various samples of biological material.	Biological Preservation,Preservation, Biologic,Biologic Preservation
D003451	Cryoprotective Agents	Substances that provide protection against the harmful effects of freezing temperatures.	Cryoprotective Agent,Cryoprotective Effect,Cryoprotective Effects,Agent, Cryoprotective,Agents, Cryoprotective,Effect, Cryoprotective,Effects, Cryoprotective
D004622	Embryo, Mammalian	The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.	Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D005615	Freezing	Liquids transforming into solids by the removal of heat.	Melting
D006018	Glycols	A generic grouping for dihydric alcohols with the hydroxy groups (-OH) located on different carbon atoms. They are viscous liquids with high boiling points for their molecular weights.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus