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Three independent approaches (binding of 125I-antibodies to polysomes, cell-free translation of polyadenylated mRNA in a wheat germ system and hybridization of RNA with a specific complementary DNA probe) were used to study the intracellular distribution of polysomes involved in the synthesis of coeruloplasmin as well as of coeruloplasmin mRNA sequences in rat liver. It was shown that only membrane-bound polysomes contain both nascent chains of coeruloplasmin and coeruloplasmin mRNA sequences. The size of coeruloplasmin polysomes is 16-18 monomers/mRNA molecule and their proportion is about 0.4-0.6% of total liver polysomes. The proportion of coeruloplasmin mRNA is 0.0025% of the total polysomal RNA and 0.29% of poly(A)-containing mRNA. These values correspond to coeruloplasmin mRNA concentration about 400-535 molecules per parenchymatous liver cell, 90% of those mRNA molecules were recovered from polysomes while the remaining 10% were from postpolysomal supernatant.
V S Gaitskhoki, and
V M L'vov, and
N K Monakhov, and
L V Puchkova, and
A L Schwartzman, and
Frolova LJu, and
N A Skobeleva, and
W Zagorski, and
S A Neifakh
January 1989,
Journal of steroid biochemistry,
V S Gaitskhoki, and
V M L'vov, and
N K Monakhov, and
L V Puchkova, and
A L Schwartzman, and
Frolova LJu, and
N A Skobeleva, and
W Zagorski, and
S A Neifakh
July 1974,
FEBS letters,
V S Gaitskhoki, and
V M L'vov, and
N K Monakhov, and
L V Puchkova, and
A L Schwartzman, and
Frolova LJu, and
N A Skobeleva, and
W Zagorski, and
S A Neifakh
August 1981,
Biokhimiia (Moscow, Russia),
V S Gaitskhoki, and
V M L'vov, and
N K Monakhov, and
L V Puchkova, and
A L Schwartzman, and
Frolova LJu, and
N A Skobeleva, and
W Zagorski, and
S A Neifakh
January 1988,
Ukrainskii biokhimicheskii zhurnal (1978),
V S Gaitskhoki, and
V M L'vov, and
N K Monakhov, and
L V Puchkova, and
A L Schwartzman, and
Frolova LJu, and
N A Skobeleva, and
W Zagorski, and
S A Neifakh
January 1991,
The Journal of steroid biochemistry and molecular biology,
V S Gaitskhoki, and
V M L'vov, and
N K Monakhov, and
L V Puchkova, and
A L Schwartzman, and
Frolova LJu, and
N A Skobeleva, and
W Zagorski, and
S A Neifakh
August 1965,
Proceedings of the National Academy of Sciences of the United States of America,
V S Gaitskhoki, and
V M L'vov, and
N K Monakhov, and
L V Puchkova, and
A L Schwartzman, and
Frolova LJu, and
N A Skobeleva, and
W Zagorski, and
S A Neifakh
January 1984,
Voprosy meditsinskoi khimii,
V S Gaitskhoki, and
V M L'vov, and
N K Monakhov, and
L V Puchkova, and
A L Schwartzman, and
Frolova LJu, and
N A Skobeleva, and
W Zagorski, and
S A Neifakh
January 1977,
Vestnik Akademii meditsinskikh nauk SSSR,
V S Gaitskhoki, and
V M L'vov, and
N K Monakhov, and
L V Puchkova, and
A L Schwartzman, and
Frolova LJu, and
N A Skobeleva, and
W Zagorski, and
S A Neifakh
January 1958,
Progress in nuclear energy. Series 6 Biological sciences,
V S Gaitskhoki, and
V M L'vov, and
N K Monakhov, and
L V Puchkova, and
A L Schwartzman, and
Frolova LJu, and
N A Skobeleva, and
W Zagorski, and
S A Neifakh
January 1981,
Molekuliarnaia biologiia,
