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A cell-free extract containing TMV-RNA replicase was prepared from TMV-infected tobacco leaves. It could synthesize double-stranded RNAs in the presence of four nucleoside triphosphates (among them, UTP was tritium-labelled), magnesium ion and actinomycin D. It was confirmed by polyacrylamide-agarose gel electrophoresis, RNase treatment, thermal denaturation and self annealing that 3H-ds RNAs, obtained from phenol-SDS extraction and Serva cellulose column chromatography, consisted of replicative form (RF) and replicative intermediate (RI) of TMV-RNA, with molecular weights of 40 X 10(6) and 5.0 X 10(6), respectively. Molecular hybridization competition experiment showed that 60-70% of the nascent RNAs in the 3H-ds RNA were plus strand of tMV-RNA.
F Rongxiang, and
W Xiaofeng, and
Q Bingyi, and
C Faxing, and
B Ming
August 1964,
Proceedings of the National Academy of Sciences of the United States of America,
F Rongxiang, and
W Xiaofeng, and
Q Bingyi, and
C Faxing, and
B Ming
March 1971,
Virology,
F Rongxiang, and
W Xiaofeng, and
Q Bingyi, and
C Faxing, and
B Ming
August 1962,
Biochemical and biophysical research communications,
F Rongxiang, and
W Xiaofeng, and
Q Bingyi, and
C Faxing, and
B Ming
March 1975,
Virology,
F Rongxiang, and
W Xiaofeng, and
Q Bingyi, and
C Faxing, and
B Ming
October 1970,
Biochemical and biophysical research communications,
F Rongxiang, and
W Xiaofeng, and
Q Bingyi, and
C Faxing, and
B Ming
October 1964,
Virology,
F Rongxiang, and
W Xiaofeng, and
Q Bingyi, and
C Faxing, and
B Ming
June 1987,
Virology,
F Rongxiang, and
W Xiaofeng, and
Q Bingyi, and
C Faxing, and
B Ming
September 1960,
Experientia,
F Rongxiang, and
W Xiaofeng, and
Q Bingyi, and
C Faxing, and
B Ming
December 1973,
Virology,
F Rongxiang, and
W Xiaofeng, and
Q Bingyi, and
C Faxing, and
B Ming
January 1963,
Voprosy virusologii,
