A new method of measuring red cell deformability and the effects of pentoxifylline. 1981

Y Isogai, and K Mochizuki, and M Ashikaga

The effect of pentoxifylline on red cell deformability was studied using a new device of a polycarbonate membrane filter ("Nuclepore') and a differential pressure type transducer. Differential pressure fluctuations due to the passage of red cells through the "Nuclepore' membrane were recorded as a differential pressure curve of red cell filtration. Red cell deformability was measured with a diluted red cell suspension (10 x 10(4)/mm3) in this buffered NaCl solution of normal osmotic pressure, 300m0sm/l as a suspending medium. Blood samples were taken from 9 healthy subjects and 26 patients with diabetes mellitus, liver disease and miscellaneous diseases. The ability of pentoxifylline to modify red cell deformability was investigated using concentrations of 20 micrograms/ml and 40 micrograms/ml in diluted red cell suspension which was incubated for 2 hours of 37 degrees C. Changes in red cell deformability were estimated from the gap between two differential pressure curves recorded in blood suspension with or without pentoxifylline. Addition of pentoxifylline increases red cell deformability in both groups of blood suspension in healthy subjects and patients. A significant increase in red cell deformability was observed in 40% of samples with the addition of 29 micrograms/ml pentoxifylline and in 77% of those with 40 micrograms/ml, respectively.

UI MeSH Term Description Entries
D008297 Male Males
D008567 Membranes, Artificial Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION. Artificial Membranes,Artificial Membrane,Membrane, Artificial
D008722 Methods A series of steps taken in order to conduct research. Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D010431 Pentoxifylline A METHYLXANTHINE derivative that inhibits phosphodiesterase and affects blood rheology. It improves blood flow by increasing erythrocyte and leukocyte flexibility. It also inhibits platelet aggregation. Pentoxifylline modulates immunologic activity by stimulating cytokine production. Agapurin,BL-191,Oxpentifylline,Pentoxil,Torental,Trental,BL 191,BL191
D011076 Polycarboxylate Cement Water-soluble low-molecular-weight polymers of acrylic or methacrylic acid that form solid, insoluble products when mixed with specially prepared ZnO powder. The resulting cement adheres to dental enamel and is also used as a luting agent. Zinc Polycarboxylate Cement,Cement, Polycarboxylate,Cement, Zinc Polycarboxylate,Polycarboxylate Cement, Zinc
D011312 Pressure A type of stress exerted uniformly in all directions. Its measure is the force exerted per unit area. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Pressures
D004912 Erythrocytes Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN. Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D005260 Female Females
D005374 Filtration A process of separating particulate matter from a fluid, such as air or a liquid, by passing the fluid carrier through a medium that will not pass the particulates. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Filtrations

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