Biomaterial Database

Inability to detect hepatitis B surface antigen (HBsAg) in the duodenum of HBsAg-positive persons. 1981

S V Feinman, and B Berris, and J C Sinclair, and S Wilson, and D Wrobel

Incubation of hepatitis B surface antigen (HBsAg)-positive serum with duodenal fluid (DF) of normal persons results in marked reduction of immunoreactivity. This was shown to be caused by: (1) interference with radioimmunoassay (RIA) system and (2) actual destruction of HBsAg. The interference in the RIA system could be counteracted by addition of bovine serum and a reproducible detection system of HBsAg in DF was established. DF of HBsAg-positive persons was negative for HBsAg. One HBsAg carrier who had a partial pancreatectomy for an insulinoma had separate cannulae in his bile and pancreatic ducts. Whereas HBsAg could be detected in bile, it was undetectable in pancreatic juice. Incubation of his pancreatic juice with his bile resulted in disappearance of HBsAg. It appears that a factor present in pancreatic juice, or resulting from the interaction of pancreatic juice and bile, results in disappearance of HBsAg immunoreactivity.

UI	MeSH Term	Description	Entries
D007419	Intestinal Secretions	Fluids originating from the epithelial lining of the intestines, adjoining exocrine glands and from organs such as the liver, which empty into the cavity of the intestines.	Intestinal Secretion,Secretion, Intestinal,Secretions, Intestinal
D010189	Pancreatic Juice	The fluid containing digestive enzymes secreted by the pancreas in response to food in the duodenum.	Juice, Pancreatic,Juices, Pancreatic,Pancreatic Juices
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D001769	Blood	The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
D004386	Duodenum	The shortest and widest portion of the SMALL INTESTINE adjacent to the PYLORUS of the STOMACH. It is named for having the length equal to about the width of 12 fingers.	Duodenums
D006500	Hepatic Duct, Common	Predominantly extrahepatic bile duct which is formed by the junction of the right and left hepatic ducts, which are predominantly intrahepatic, and, in turn, joins the cystic duct to form the common bile duct.	Common Hepatic Duct,Hepatic Duct,Common Hepatic Ducts,Duct, Common Hepatic,Duct, Hepatic,Ducts, Common Hepatic,Ducts, Hepatic,Hepatic Ducts,Hepatic Ducts, Common
D006514	Hepatitis B Surface Antigens	Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.	Australia Antigen,HBsAg,Hepatitis B Surface Antigen,Antigen, Australia
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001646	Bile	An emulsifying agent produced in the LIVER and secreted into the DUODENUM. Its composition includes BILE ACIDS AND SALTS; CHOLESTEROL; and ELECTROLYTES. It aids DIGESTION of fats in the duodenum.	Biliary Sludge,Sludge, Biliary
D012463	Saliva	The clear, viscous fluid secreted by the SALIVARY GLANDS and mucous glands of the mouth. It contains MUCINS, water, organic salts, and ptylin.	Salivas