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Biosynthesis of ceruloplasmin was studied in wheat germ extract programmed with polysomal RNA from rat liver. Optimal potassium concentration for the total protein-synthesizing activity and for the synthesis of immunoreactive ceruloplasmin was 96 and 186 mM respectively. 7-methylguanosine 5'-monophosphate caused two-fold inhibition of the cell-free synthesis of ceruloplasmin. Immunoprecipitated ceruloplasmin that was synthesized at optimal potassium concentration was a homogeneous polypeptide of a molecular weight about 84 kD. The addition of membrane fractions from rat liver to the incubation mixture caused the conversion of the 84 kD polypeptide into 80 kD and 65 kD polypeptides that are similar to proceruloplasmins synthesized in rat liver during in vivo pulse labelling. The suggestion is made that 84 kD polypeptide is a primary product of the translation of ceruloplasmin mRNA (preproceruloplasmin).
L V Puchkova, and
V S Gaitskhoki, and
N K Monakhov, and
L T Timchenko, and
S A Neifakh
May 1976,
The Journal of biological chemistry,
L V Puchkova, and
V S Gaitskhoki, and
N K Monakhov, and
L T Timchenko, and
S A Neifakh
December 1976,
Proceedings of the National Academy of Sciences of the United States of America,
L V Puchkova, and
V S Gaitskhoki, and
N K Monakhov, and
L T Timchenko, and
S A Neifakh
November 1973,
Journal of virology,
L V Puchkova, and
V S Gaitskhoki, and
N K Monakhov, and
L T Timchenko, and
S A Neifakh
May 1980,
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L V Puchkova, and
V S Gaitskhoki, and
N K Monakhov, and
L T Timchenko, and
S A Neifakh
January 1975,
Cold Spring Harbor symposia on quantitative biology,
L V Puchkova, and
V S Gaitskhoki, and
N K Monakhov, and
L T Timchenko, and
S A Neifakh
December 1973,
Virology,
L V Puchkova, and
V S Gaitskhoki, and
N K Monakhov, and
L T Timchenko, and
S A Neifakh
June 1983,
FEBS letters,
L V Puchkova, and
V S Gaitskhoki, and
N K Monakhov, and
L T Timchenko, and
S A Neifakh
December 1975,
European journal of biochemistry,
L V Puchkova, and
V S Gaitskhoki, and
N K Monakhov, and
L T Timchenko, and
S A Neifakh
December 1982,
Acta embryologiae et morphologiae experimentalis ("Halocynthia" Association"),
L V Puchkova, and
V S Gaitskhoki, and
N K Monakhov, and
L T Timchenko, and
S A Neifakh
July 1978,
Proceedings of the National Academy of Sciences of the United States of America,
