Biomaterial Database

Purification and characterization of phosphoglycerate kinase from Fasciola hepatica. 1981

M D Schulman, and D Valentino

Phosphoglycerate kinase (EC 2.7.2.3) of Fasciola hepatica was purified 375-fold to homogeneity. The enzyme was monomeric, and had a molecular weight of 47 900 and a sedimentation coefficient of 3.0-3.5 S. The enzyme was composed of 397 amino acids and was relatively rich in sulfur amino acids containing 13 methionine and 2 cysteine residues per mole. The enzyme possessed a highly reactive essential sulfhydryl group and was inhibited irreversibly by iodoacetamide and N-ethylmaleimide and reversibly by p-chloromercuribenzoate and 5,5'-dithio-bis(2-nitrobenzoic acid). Initial velocity studies suggested that reaction occurred via a sequential mechanism. The Km values for 3-phosphoglycerate and ATP were 1.26 and 0.90 mM, respectively. ADP was a noncompetitive inhibitor with respect to both ATP and 3-phosphoglycerate.

UI	MeSH Term	Description	Entries
D008433	Mathematics	The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Mathematic
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D010735	Phosphoglycerate Kinase	An enzyme catalyzing the transfer of a phosphate group from 3-phospho-D-glycerate in the presence of ATP to yield 3-phospho-D-glyceroyl phosphate and ADP. EC 2.7.2.3.	Kinase, Phosphoglycerate
D005210	Fasciola hepatica	A species of helminth commonly called the sheep liver fluke. It occurs in the biliary passages, liver, and gallbladder during various stages of development. Snails and aquatic vegetation are the intermediate hosts. Occasionally seen in man, it is most common in sheep and cattle.	Liver Fluke,Fasciola hepaticas,Fluke, Liver,Flukes, Liver,Liver Flukes,hepatica, Fasciola
D000596	Amino Acids	Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.	Amino Acid,Acid, Amino,Acids, Amino
D013438	Sulfhydryl Compounds	Compounds containing the -SH radical.	Mercaptan,Mercapto Compounds,Sulfhydryl Compound,Thiol,Thiols,Mercaptans,Compound, Sulfhydryl,Compounds, Mercapto,Compounds, Sulfhydryl