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The influence of alloxan on mammalian cell glucose metabolism has been investigated using human diploid fibroblastic cells in culture. When cell monolayers were exposed to D-[14C]glucose, the presence of alloxan (0.31-1.87 mM) resulted initially in a dose-dependent enhancement of total cell glucose incorporation. This was observed within 2 min and declined by 6 min. After that time, alloxan inhibited glucose incorporation. When hexose transport was examined directly using glucose analogues, alloxan neither enhanced nor inhibited the uptake of 3-O-methylglucose or 2-deoxyglucose. Alloxan exerted no effect on cell permeability or cell viability. These results suggest that alloxan may directly influence cell glucose metabolism beyond the level of phosphorylation. The dual effect of alloxan on glucose incorporation may be related to the alloxan stimulation and subsequent inhibition of glucose-induced insulin release in pancreatic islets.
F Ishibashi, and
H Hidaka, and
R M Fields, and
B V Howard, and
P H Bennett
December 1981,
The Journal of biological chemistry,
F Ishibashi, and
H Hidaka, and
R M Fields, and
B V Howard, and
P H Bennett
June 1981,
The American journal of physiology,
F Ishibashi, and
H Hidaka, and
R M Fields, and
B V Howard, and
P H Bennett
January 1980,
Biochimica et biophysica acta,
F Ishibashi, and
H Hidaka, and
R M Fields, and
B V Howard, and
P H Bennett
April 1983,
Biochimica et biophysica acta,
F Ishibashi, and
H Hidaka, and
R M Fields, and
B V Howard, and
P H Bennett
October 1979,
Journal of cellular physiology,
F Ishibashi, and
H Hidaka, and
R M Fields, and
B V Howard, and
P H Bennett
May 1982,
Metabolism: clinical and experimental,
F Ishibashi, and
H Hidaka, and
R M Fields, and
B V Howard, and
P H Bennett
September 1972,
The Journal of biological chemistry,
F Ishibashi, and
H Hidaka, and
R M Fields, and
B V Howard, and
P H Bennett
April 1978,
Journal of cellular physiology,
F Ishibashi, and
H Hidaka, and
R M Fields, and
B V Howard, and
P H Bennett
January 1964,
Studii si cercetari de medicina interna,
F Ishibashi, and
H Hidaka, and
R M Fields, and
B V Howard, and
P H Bennett
October 1994,
Biochemistry and molecular biology international,
