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The in vitro metabolism of the anticancer agent 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has been studied in male Fischer 344 rat liver microsomal preparations. The previously identified product. 1,3-bis(2-chloroethyl)urea (BCU), has been shown to be the major metabolite. Stable isotope labeling and mass spectral analysis of isolated metabolites indicate that BCU is formed exclusively from the metabolic denitrosation of BCNU. The rate of BCNU chemical decomposition in rat liver microsomal preparations deficient in NADPH and the metabolic disappearance rate in preparations containing added NADPH were measured and compared with the measured rate of metabolic formation of BCU under the same conditions. The rate of NADPH-dependent BCNU metabolism and BCU formation are equal within experimental error. BCNU was found to inhibit the rat liver 9000 g supernatant metabolism of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU).
H S Lin, and
R J Weinkam
June 1995,
Mutation research,
H S Lin, and
R J Weinkam
January 1965,
Antimicrobial agents and chemotherapy,
H S Lin, and
R J Weinkam
February 1979,
Cancer research,
H S Lin, and
R J Weinkam
December 1980,
Neurosurgery,
H S Lin, and
R J Weinkam
December 1975,
Biochemistry,
H S Lin, and
R J Weinkam
July 1965,
Applied microbiology,
H S Lin, and
R J Weinkam
January 1965,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
H S Lin, and
R J Weinkam
February 1974,
Cancer research,
H S Lin, and
R J Weinkam
January 1988,
European journal of biochemistry,
H S Lin, and
R J Weinkam
February 1973,
American journal of obstetrics and gynecology,
