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The synthesis of a set of protected ribonucleosides bearing a 5'-monomethoxytrityl group and a 2'-t-butyldimethylsilyl group (series 1, MMTNSiOH) and another set having a 2'-t-butyldimethylsilyl and a 3'-levulinyl group (series 3, HONSiLV) is described. These two sets of derivatives along with the dichlorophosphite condensation procedure lead to a rapid and versatile synthetic procedure for the preparation of oligoribonucleotides. Nucleotide chains possessing a methoxytrityl group at the 5'-end and a levulynyl group at the 3'-end possess an easily detected PMR label at each end (a distinct methyl singlet). The synthetic method is illustrated by the synthesis of a hexadecauridylic acid by a block condensation procedure.
K K Ogilvie, and
M J Nemer, and
N Thériault, and
R Pon, and
J M Seifert
January 1990,
Nucleic acids symposium series,
K K Ogilvie, and
M J Nemer, and
N Thériault, and
R Pon, and
J M Seifert
January 1983,
Nucleic acids symposium series,
K K Ogilvie, and
M J Nemer, and
N Thériault, and
R Pon, and
J M Seifert
January 1980,
Nucleic acids symposium series,
K K Ogilvie, and
M J Nemer, and
N Thériault, and
R Pon, and
J M Seifert
January 2013,
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K K Ogilvie, and
M J Nemer, and
N Thériault, and
R Pon, and
J M Seifert
April 1994,
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K K Ogilvie, and
M J Nemer, and
N Thériault, and
R Pon, and
J M Seifert
January 2000,
Nucleosides, nucleotides & nucleic acids,
K K Ogilvie, and
M J Nemer, and
N Thériault, and
R Pon, and
J M Seifert
October 1990,
The Journal of organic chemistry,
K K Ogilvie, and
M J Nemer, and
N Thériault, and
R Pon, and
J M Seifert
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K K Ogilvie, and
M J Nemer, and
N Thériault, and
R Pon, and
J M Seifert
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K K Ogilvie, and
M J Nemer, and
N Thériault, and
R Pon, and
J M Seifert
May 1989,
Nucleic acids research,
