Biomaterial Database

Na+-dependent co-transport of alpha-methyl D-glucoside across the mucosal border of rabbit descending colon. 1981

A Ilundain, and R J Naftalin

(1) The uptake and bidirectional fluxes of 1-alpha-methyl D-glucoside were studied in isolated rabbit colonic mucosa. (2) The uptake of alpha-methyl D-glucoside was linear over the first 30 min and reached maximum after 1 h; was a saturable function of sugar concentration and was Na+-dependent. (3) An increase in sugar uptake across the mucosal border and net transepithelial sugar flux across sheets of colon was observed in the presence of 10(-4) M amiloride. (4) Phlorizin (10(-4) M) inhibited sugar uptake into the tissue water and abolished net sugar flux. Amiloride-stimulated sugar uptake was also abolished by 10(-4) M phlorizin. (5) Ouabain (10(-4) M) prevented the effect of amiloride on sugar uptake and inhibited sugar uptake into the tissue. (6) These results corroborate the findings of Henriques de Jesus et al. (Henriques de Jesus, C., Da Gracia Emilio, M. and Santos, M.A. Gastroenterol. Clin. Biol. 3, 172-173) who found a sugar-dependent increase in short-circuit current in colonic mucosa exposed to amiloride.

UI	MeSH Term	Description	Entries
D007082	Ileum	The distal and narrowest portion of the SMALL INTESTINE, between the JEJUNUM and the ILEOCECAL VALVE of the LARGE INTESTINE.
D007413	Intestinal Mucosa	Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.	Intestinal Epithelium,Intestinal Glands,Epithelium, Intestinal,Gland, Intestinal,Glands, Intestinal,Intestinal Gland,Mucosa, Intestinal
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008297	Male		Males
D008757	Methylglucosides		Methylglucopyranosides
D008759	Methylglycosides
D009928	Organ Specificity	Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen.	Tissue Specificity,Organ Specificities,Specificities, Organ,Specificities, Tissue,Specificity, Organ,Specificity, Tissue,Tissue Specificities
D010042	Ouabain	A cardioactive glycoside consisting of rhamnose and ouabagenin, obtained from the seeds of Strophanthus gratus and other plants of the Apocynaceae; used like DIGITALIS. It is commonly used in cell biological studies as an inhibitor of the NA(+)-K(+)-EXCHANGING ATPASE.	Acocantherin,G-Strophanthin,Acolongifloroside K,G Strophanthin
D011092	Polyethylene Glycols	Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.	Macrogols,Polyoxyethylenes,Carbowax,Macrogol,Polyethylene Glycol,Polyethylene Oxide,Polyethyleneoxide,Polyglycol,Glycol, Polyethylene,Glycols, Polyethylene,Oxide, Polyethylene,Oxides, Polyethylene,Polyethylene Oxides,Polyethyleneoxides,Polyglycols,Polyoxyethylene
D011817	Rabbits	A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes.	Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus