-transparent.png){kind=logo}
Hybridoma cell lines secreting monoclonal antibodies to carcinoembryonic antigen (CEA) were generated by fusing mouse immune lymphocytes with the mouse myeloma variant cell line, NS-1. Antibody secreted by one cloned hybrid cell line could bind only a select portion of the CEA bound by the commercially available goat anti-CEA antiserum used in clinical assays. Radiolabeled CEA could be purified on a monoclonal antibody affinity column. Incorporation of this purified radiolabeled CEA in a double-antibody solid-phase assay with goat anti-CEA antiserum led to an approximately 2.5-fold increase in sensitivity of the assay. Genetically stable hybrid clones may be sources of virtually unlimited quantities of such antibodies which may have potential utility in improving the cancer specificity of clinical assays.
H Z Kupchik, and
V R Zurawski, and
J G Hurrell, and
N Zamcheck, and
P H Black
January 1995,
ILAR journal,
H Z Kupchik, and
V R Zurawski, and
J G Hurrell, and
N Zamcheck, and
P H Black
February 1984,
Taiwan yi xue hui za zhi. Journal of the Formosan Medical Association,
H Z Kupchik, and
V R Zurawski, and
J G Hurrell, and
N Zamcheck, and
P H Black
January 1986,
Medicina,
H Z Kupchik, and
V R Zurawski, and
J G Hurrell, and
N Zamcheck, and
P H Black
January 1980,
Proceedings of the National Academy of Sciences of the United States of America,
H Z Kupchik, and
V R Zurawski, and
J G Hurrell, and
N Zamcheck, and
P H Black
February 1981,
Gastroenterology,
H Z Kupchik, and
V R Zurawski, and
J G Hurrell, and
N Zamcheck, and
P H Black
October 1990,
Thrombosis and haemostasis,
H Z Kupchik, and
V R Zurawski, and
J G Hurrell, and
N Zamcheck, and
P H Black
March 1988,
Immunological investigations,
H Z Kupchik, and
V R Zurawski, and
J G Hurrell, and
N Zamcheck, and
P H Black
January 1984,
La Ricerca in clinica e in laboratorio,
H Z Kupchik, and
V R Zurawski, and
J G Hurrell, and
N Zamcheck, and
P H Black
February 1983,
Cancer research,
H Z Kupchik, and
V R Zurawski, and
J G Hurrell, and
N Zamcheck, and
P H Black
January 1990,
Natural immunity and cell growth regulation,
