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We have developed a sensitive double antibody radioimmunoassay for measuring canine cardiac cathepsin D. Radioiodinated cathepsin D was prepared by chloramine T oxidation using a highly purified source of enzyme. High avidity antiserum to the canine cardiac enzyme was raised in rabbits. Antibody-bound cathepsin D was separated from free enzyme using goat anti-rabbit IgG second antibody. The least amount of immunoreactive enzyme measurable in the radioimmunoassay was 2.4 ng.cm-3 as determined by antibody titration. The assay was linear for concentrations of enzyme in the range of 10 to 120 ng.cm-3. Within-assay and between-assay variations were 12%. The radioimmunoassay described was used to measure the immunoreactive cathepsin D content of the 100 000 x g supernatant fraction of canine myocardial homogenates.
A M Samarel, and
E A Ogunro, and
A G Ferguson, and
M Lesch
November 1972,
American journal of veterinary research,
A M Samarel, and
E A Ogunro, and
A G Ferguson, and
M Lesch
January 1981,
Methods in enzymology,
A M Samarel, and
E A Ogunro, and
A G Ferguson, and
M Lesch
May 1980,
Cardiovascular research,
A M Samarel, and
E A Ogunro, and
A G Ferguson, and
M Lesch
January 1974,
The Journal of laboratory and clinical medicine,
A M Samarel, and
E A Ogunro, and
A G Ferguson, and
M Lesch
August 1990,
Clinica chimica acta; international journal of clinical chemistry,
A M Samarel, and
E A Ogunro, and
A G Ferguson, and
M Lesch
June 2001,
Immunology letters,
A M Samarel, and
E A Ogunro, and
A G Ferguson, and
M Lesch
October 1975,
Applied microbiology,
A M Samarel, and
E A Ogunro, and
A G Ferguson, and
M Lesch
March 1974,
Journal of the National Cancer Institute,
A M Samarel, and
E A Ogunro, and
A G Ferguson, and
M Lesch
January 1977,
Clinical chemistry,
A M Samarel, and
E A Ogunro, and
A G Ferguson, and
M Lesch
April 1978,
Canadian journal of microbiology,
