Biomaterial Database

[Effect of the alkylating agent Trenimon on the cell membrane]. 1981

S Walliser

The alkylating agent Trenimon (10(-9) mol/l - 10(-6) mol/l) caused characteristic changes in membrane polarization, in cell counts and in surface charge (zeta potential). After short incubation time this effect of Trenimon is particularly pronounced in terms of transmembrane potential (TMP), whereas a decrease in surface charge was found only after incubation times longer than 24 hours. Immediately after addition of the chemotherapeutic agent to the HeLa cells damped oscillation of the TMP was observed with amplitudes being a function of the concentration of the agent. For incubation times between 24 and 96 hours low concentration (10(-8) mol/l) entailed increase in membrane polarization. Membrane depolarization was enhanced at higher doses of 10(-7) mol/l and 10(-6) mol/l. After lengthy incubation time drastic depolarizations and decrease in surface charge were accompanied by morphological cell damage such as "giant cells" and "off spherical" cells. Grunicke et al. [4] also found after Trenimon administration changes in membrane function before the DNA synthesis was influenced. These observations indicate that the cell membrane can be considered as a direct point for the effect of the alkylating agent Trenimon.

UI	MeSH Term	Description	Entries
D008564	Membrane Potentials	The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).	Resting Potentials,Transmembrane Potentials,Delta Psi,Resting Membrane Potential,Transmembrane Electrical Potential Difference,Transmembrane Potential Difference,Difference, Transmembrane Potential,Differences, Transmembrane Potential,Membrane Potential,Membrane Potential, Resting,Membrane Potentials, Resting,Potential Difference, Transmembrane,Potential Differences, Transmembrane,Potential, Membrane,Potential, Resting,Potential, Transmembrane,Potentials, Membrane,Potentials, Resting,Potentials, Transmembrane,Resting Membrane Potentials,Resting Potential,Transmembrane Potential,Transmembrane Potential Differences
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D006367	HeLa Cells	The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays.	Cell, HeLa,Cells, HeLa,HeLa Cell
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014228	Triaziquone	Alkylating antineoplastic agent used mainly for ovarian tumors. It is toxic to skin, gastrointestinal tract, bone marrow and kidneys.	2,3,5-Tris(ethyleneimine)benzoquinone,Trenimon