Biomaterial Database

Comparison of three serum alpha-hydroxybutyrate dehydrogenase procedures for lactate dehydrogenase isoenzyme discrimination. 1981

F Y Leung, and A R Henderson

Three recently proposed methods for the assay of alpha-hydroxybutyrate (2-oxobutyrate) dehydrogenase (HBD) activity in serum were assessed to determine which one would give the most useful discrimination between the lactate dehydrogenase (LD) isoenzymes. The 2-oxobutyrate concentration and temperature for these procedures were 3.3 mmol/l at 30 degree C, 12 mmol/l at 30 degree C and 15 mmol/l at 37 degree C. LD isoenzyme activity values obtained by thin-layer agarose electrophoresis were compared with HBD activity results by linear regression analysis. The best, statistically significant, correlation of LD-1 with HBD activities was obtained with 3.3 mmol/l at 30 degree C (regression coefficient, 0.92; correlation coefficient, 0.98).

UI	MeSH Term	Description	Entries
D007231	Infant, Newborn	An infant during the first 28 days after birth.	Neonate,Newborns,Infants, Newborn,Neonates,Newborn,Newborn Infant,Newborn Infants
D007527	Isoenzymes	Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.	Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D007770	L-Lactate Dehydrogenase	A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.	Lactate Dehydrogenase,Dehydrogenase, L-Lactate,Dehydrogenase, Lactate,L Lactate Dehydrogenase
D004587	Electrophoresis, Agar Gel	Electrophoresis in which agar or agarose gel is used as the diffusion medium.	Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006884	Hydroxybutyrate Dehydrogenase		3-Hydroxybutyrate Dehydrogenase,D-3-Hydroxybutyrate Dehydrogenase,D-beta-Hydroxybutyrate Dehydrogenase,3 Hydroxybutyrate Dehydrogenase,D 3 Hydroxybutyrate Dehydrogenase,D beta Hydroxybutyrate Dehydrogenase,Dehydrogenase, 3-Hydroxybutyrate,Dehydrogenase, D-3-Hydroxybutyrate,Dehydrogenase, D-beta-Hydroxybutyrate,Dehydrogenase, Hydroxybutyrate
D013379	Substrate Specificity	A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.	Specificities, Substrate,Specificity, Substrate,Substrate Specificities