BIOMAT Database Logo BIOMAT Database Logo

Enzyme-linked immunosorbent assay for human IgG, IgA, and IgM antibodies to antigens from anaerobic cultures of seven oral bacteria. 1981

K Tolo, and K Schenck, and P Brandtzaeg

Monocultures of 7 oral bacteria were grown anaerobically, and antigens were partially purified from the supernatant fluids by gel filtration. Isoelectric focussing showed that the antigen fractions contained PAS-positive material and proteins focussing between pH 3.5 and pH 5.5. Specific activity against the 7 antigen fractions was observed for IgG, IgA, and IgM in serum from patients with periodontal disease by means of enzyme-linked immunosorbent assay (ELISA). Conditions for antigen coating in ELISA were studied, and large variations with regard to optimal concentration were found among the 7 fractions. The ELISA readings were virtually unaffected by increasing the pH of the coating buffer from 5 to 9. Addition of sheep serum to the incubation buffer was disadvantageous as the serum showed moderate antibody activity to the 7 antigen fractions, and human serum apparently contained IgM antibodies to sheep IgG. Addition of 0.5% BSA improved the reproducibility of ELISA considerably, although the sensitivity was somewhat decreased.

UI MeSH Term Description Entries
D007070 Immunoglobulin A Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions. IgA,IgA Antibody,IgA1,IgA2,Antibody, IgA
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007075 Immunoglobulin M A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally was called a macroglobulin. Gamma Globulin, 19S,IgM,IgM Antibody,IgM1,IgM2,19S Gamma Globulin,Antibody, IgM
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D004306 Dose-Response Relationship, Immunologic A specific immune response elicited by a specific dose of an immunologically active substance or cell in an organism, tissue, or cell. Immunologic Dose-Response Relationship,Relationship, Immunologic Dose-Response,Dose Response Relationship, Immunologic,Dose-Response Relationships, Immunologic,Immunologic Dose Response Relationship,Immunologic Dose-Response Relationships,Relationship, Immunologic Dose Response,Relationships, Immunologic Dose-Response
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005673 Fusobacterium A genus of gram-negative, anaerobic, rod-shaped bacteria found in cavities of humans and other animals. No endospores are formed. Some species are pathogenic and occur in various purulent or gangrenous infections. Sphaerophorus
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000693 Anaerobiosis The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Anaerobic Metabolism,Anaerobic Metabolisms,Anaerobioses,Metabolism, Anaerobic,Metabolisms, Anaerobic

Related Publications

K Tolo, and K Schenck, and P Brandtzaeg
Enzyme-linked immunosorbent assay for subclass distribution of human IgG and IgA antigen-specific antibodies.
April 1985, Journal of immunological methods,
K Tolo, and K Schenck, and P Brandtzaeg
Enzyme-linked immunosorbent assay (ELISA) for IgM, IgG and IgA class antibodies against Candida albicans antigens: development and comparison with other methods.
June 1981, Sabouraudia,
K Tolo, and K Schenck, and P Brandtzaeg
IgG/IgA pemphigus with IgG and IgA antidesmoglein 1 antibodies detected by enzyme-linked immunosorbent assay.
November 2002, The British journal of dermatology,
K Tolo, and K Schenck, and P Brandtzaeg
An enzyme-linked immunosorbent assay for the detection of IgG and IgM antibodies to human herpesvirus type 6.
March 1993, Journal of virological methods,
K Tolo, and K Schenck, and P Brandtzaeg
Enzyme-linked immunosorbent assay for detection of IgG and IgM class antibodies to Japanese encephalitis virus.
December 1983, The Kobe journal of medical sciences,
K Tolo, and K Schenck, and P Brandtzaeg
An enzyme-linked immunosorbent assay for the detection of IgG, IgA and IgM antibodies to rinderpest virus in experimentally infected cattle.
March 1982, Research in veterinary science,
K Tolo, and K Schenck, and P Brandtzaeg
Determination of IgG, IgM and IgA antibody responses in human toxoplasmosis by enzyme-linked immunosorbent assay (ELISA).
January 1983, Scandinavian journal of infectious diseases,
K Tolo, and K Schenck, and P Brandtzaeg
IgG antibodies to purified Aspergillus fumigatus antigens determined by enzyme-linked immunosorbent assay.
January 1984, International archives of allergy and applied immunology,
K Tolo, and K Schenck, and P Brandtzaeg
Enzyme-linked immunosorbent assay (ELISA) for IgG antibodies to thyroglobulin.
February 1987, Clinica chimica acta; international journal of clinical chemistry,
K Tolo, and K Schenck, and P Brandtzaeg
[Enzyme-linked immunosorbent assay (ELISA) for IgG- and IgM antibodies in chlamydial infections of humans].
November 1983, Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,
Copied contents to your clipboard!