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Isolated rat liver DNA was fractionated on benzoylated-naphthoylated-DEAE-cellulose (BND-cellulose) columns. In experiments with dimethyl-nitrosamine (DMN)-treated (1-30 mg/kg) rats killed 24 h after injection, the amount of DNA eluting in the formamide fraction (i.e., containing single-stranded or partially denatured regions) was increased in a dose-dependent way. The absence of an effect at 6 days after DMN indicated that above regions disappear much more rapidly from rat liver DNA than do those DMN-induced lesions that give rise to more persistent DNA fragmentation in alkaline sucrose gradients. This indicates that 2 different lesions are involved. When the extents of alkylation at the O6- and 7-position of guanine in the NaCl-eluted and formamide-eluted DNA were compared, no significant differences were observed. Our results indicate that, at least in the present system, neither the presence nor the repair of O6-alkylguanine is related to the formation of single-stranded regions in rat liver DNA.
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