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Explants of male Xenopus liver maintained in a serum-free culture medium respond to stimulation by 2 X 10(-8) M 17beta-estradiol with an increasing rate of accumulation of vitellogenin mRNA, as revealed by hybridization of cDNA to the total cytoplasmic RNA extracted from the cultures. A similar response is observed for secretion of 32PO4-labeled vitellogenin into the culture medium. The in vitro response is improved in liver tissue of prestimulated animals, and by adaptation of liver explants to the culture medium prior to hormone treatment, but attains only about 10% of the in vivo response. Since essential features of the in vivo response are maintained in liver explants, organ culture appears suitable for investigating initial events of estradiol action leading to enhanced synthesis of vitellogenin.
B K Felber, and
G U Ryffel, and
R Weber
January 1976,
Cell,
B K Felber, and
G U Ryffel, and
R Weber
June 1981,
Biochimica et biophysica acta,
B K Felber, and
G U Ryffel, and
R Weber
February 1982,
Developmental biology,
B K Felber, and
G U Ryffel, and
R Weber
July 1976,
European journal of biochemistry,
B K Felber, and
G U Ryffel, and
R Weber
February 1985,
Molecular and cellular endocrinology,
B K Felber, and
G U Ryffel, and
R Weber
November 1976,
Biochimica et biophysica acta,
B K Felber, and
G U Ryffel, and
R Weber
December 1978,
Nucleic acids research,
B K Felber, and
G U Ryffel, and
R Weber
June 1978,
Nature,
B K Felber, and
G U Ryffel, and
R Weber
March 1978,
European journal of biochemistry,
B K Felber, and
G U Ryffel, and
R Weber
August 1975,
Proceedings of the National Academy of Sciences of the United States of America,
