Biomaterial Database

Alkaline phosphatase activity in lymph and serum of bile duct-ligated rats. 1978

A L Baker, and S C Hauser

To determine whether liver alkaline phosphatase (AP) reaches the circulation by crossing the space of Dissé and traversing lymphatic channels to the thoracic duct, AP was measured in lymph and serum hourly for 12 h after bile duct ligation in thoracic duct cannulated rats. AP did not rise at any time point in lymph, was markedly increased in serum by 7 h when compared with controls (p less than 0.05), and continued to rise for the next 5 h. However, AP in the thoracic duct lymph was increased 5-fold by corn oil feeding. Molecular weight for serum AP from bile duct-ligated rats was determined by gel chromatography and found to be approximately 125,000. Thus, hepatic AP molecules probably pass freely through the large openings in the sinusoidal endothelial lining and are transported out of the liver chiefly in sinusoidal blood, which flows more rapidly than liver lymph.

UI	MeSH Term	Description	Entries
D008026	Ligation	Application of a ligature to tie a vessel or strangulate a part.	Ligature,Ligations,Ligatures
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008196	Lymph	The interstitial fluid that is in the LYMPHATIC SYSTEM.	Lymphs
D008297	Male		Males
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D009821	Oils	Unctuous combustible substances that are liquid or easily liquefiable on warming, and are soluble in ether but insoluble in water. Such substances, depending on their origin, are classified as animal, mineral, or vegetable oils. Depending on their behavior on heating, they are volatile or fixed. (Dorland, 28th ed)
D000469	Alkaline Phosphatase	An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001652	Bile Ducts	The channels that collect and transport the bile secretion from the BILE CANALICULI, the smallest branch of the BILIARY TRACT in the LIVER, through the bile ductules, the bile ducts out the liver, and to the GALLBLADDER for storage.	Bile Duct,Duct, Bile,Ducts, Bile
D013897	Thoracic Duct	The largest lymphatic vessel that passes through the chest and drains into the SUBCLAVIAN VEIN.	Cisterna Chyli,Chyli, Cisterna,Chylus, Cisterna,Cisterna Chylus,Duct, Thoracic,Ducts, Thoracic,Thoracic Ducts