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Hepatocytes isolated from female rats meal-fed a high-glucose diet were incubated in Krebs-Henseleit bicarbonate medium containing 16.5 mM glucose, 3H2O, and 14C-labeled amino acids (-)-Hydroxycitrate depressed the incorporation of 3H2O and [14C] alanine into fatty acids and cholesterol. Incorporation of [U-14C]leucine into lipids was not affected but incorporation of 3H2O into lipids was decreased significantly by (-)-hydroxycitrate. (-)-Hydroxycitrate depressed the incorporation of radioactivity from [2-14C]leucine into fatty acids and cholesterol by 61 and 38%, respectively, and stimulated the incorporation of radioactivity from [4,5-3H]leucine 35 and 28%. As [2-14C]leucine labels the acetyl-CoA pool and [4,5-3H]leucine labels the acetoacetate pool, it was concluded that mitochondrial 3-hydroxy-3-methylglutaryl-CoA is not incorporated intact into cholesterol, and that acetoacetate can be activated effectively in the liver cytosol for support of cholesterol and fatty acid synthesis.
M M Mathias, and
A C Sullivan, and
J G Hamilton
October 1977,
Lipids,
M M Mathias, and
A C Sullivan, and
J G Hamilton
October 1991,
Biochemical pharmacology,
M M Mathias, and
A C Sullivan, and
J G Hamilton
March 1998,
Biochemistry and molecular biology international,
M M Mathias, and
A C Sullivan, and
J G Hamilton
November 1985,
Archives internationales de physiologie et de biochimie,
M M Mathias, and
A C Sullivan, and
J G Hamilton
August 1995,
The British journal of nutrition,
M M Mathias, and
A C Sullivan, and
J G Hamilton
January 1982,
The International journal of biochemistry,
M M Mathias, and
A C Sullivan, and
J G Hamilton
November 1983,
FEBS letters,
M M Mathias, and
A C Sullivan, and
J G Hamilton
September 1986,
Biochemical and biophysical research communications,
M M Mathias, and
A C Sullivan, and
J G Hamilton
March 1982,
The Biochemical journal,
M M Mathias, and
A C Sullivan, and
J G Hamilton
August 1978,
The Journal of laboratory and clinical medicine,
