Biomaterial Database

Effect of topical 16,16-dimethyl prostaglandin E2 on aspirin-induced disruption of gastric permeability barrier in dogs. 1981

L Y Cheung

This study was designed to evaluate whether stimulation of mucus and alkaline secretion by topical 16,16-dimethyl prostaglandin E2 (dmPGE2) would protect gastric mucosa against aspirin-induced disruption of permeability barrier to lumina acid. A chambered segment of canine fundic stomach with intact vascular supply was partitioned into two compartments. One was pretreated with topical dmPGE2 (25 micrograms/15 ml isotonic acid) and the other served as control. Topical dmPGE2 resulted in 1) mucus release as evidenced by gross and microscopic examinations, 2) luminal volume gain from 0.05 +/- 0.01 to 1.05 +/- 0.30 ml/15 min (p less than 0.01) and 3) an increase in Na+ efflux from 9 +/- 4 to 165 +/- 20 muEq/15 min (p less than 0.001). These effects of dmPGE2 did not protect the gastric mucosa against disruption of permeability barrier induced by 20 mM aspirin, which resulted in a similar amount of H+ back-diffusion in dmPGE2-treated mucosa (8 +/- 7 to 129 +/- 8 muEq/15 min, p less than 0.001) and in control mucosa (from 5 +/- 7 to 118 +/- 24 muEq/15 min, p less than 0.001). Also there was no significant difference in the degree of reduction in mucosal potential difference between dmPGE2-treated mucosa (from 60 +/- 4 to 34 +/- 2 -mV p less than 0.01) and control mucosa (from 58 +/- 4 to 36 +/- 3 -mV p less than 0.001).

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D010539	Permeability	Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions.	Permeabilities
D011459	Prostaglandins E, Synthetic	Analogs or derivatives of prostaglandins E that do not occur naturally in the body. They do not include the product of the chemical synthesis of hormonal PGE.	PGE Synthetic,Prostaglandin E Analogs,Prostaglandin E Analogues,Synthetic Prostaglandins E,Analogs, Prostaglandin E,Analogues, Prostaglandin E,Synthetic, PGE
D004285	Dogs	The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)	Canis familiaris,Dog
D005750	Gastric Juice	The liquid secretion of the stomach mucosa consisting of hydrochloric acid (GASTRIC ACID); PEPSINOGENS; INTRINSIC FACTOR; GASTRIN; MUCUS; and the bicarbonate ion (BICARBONATES). (From Best & Taylor's Physiological Basis of Medical Practice, 12th ed, p651)	Gastric Juices,Juice, Gastric,Juices, Gastric
D005753	Gastric Mucosa	Lining of the STOMACH, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. The surface cells produce MUCUS that protects the stomach from attack by digestive acid and enzymes. When the epithelium invaginates into the LAMINA PROPRIA at various region of the stomach (CARDIA; GASTRIC FUNDUS; and PYLORUS), different tubular gastric glands are formed. These glands consist of cells that secrete mucus, enzymes, HYDROCHLORIC ACID, or hormones.	Cardiac Glands,Gastric Glands,Pyloric Glands,Cardiac Gland,Gastric Gland,Gastric Mucosas,Gland, Cardiac,Gland, Gastric,Gland, Pyloric,Glands, Cardiac,Glands, Gastric,Glands, Pyloric,Mucosa, Gastric,Mucosas, Gastric,Pyloric Gland
D000287	Administration, Topical	The application of drug preparations to the surfaces of the body, especially the skin (ADMINISTRATION, CUTANEOUS) or mucous membranes. This method of treatment is used to avoid systemic side effects when high doses are required at a localized area or as an alternative systemic administration route, to avoid hepatic processing for example.	Drug Administration, Topical,Administration, Topical Drug,Topical Administration,Topical Drug Administration,Administrations, Topical,Administrations, Topical Drug,Drug Administrations, Topical,Topical Administrations,Topical Drug Administrations
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001241	Aspirin	The prototypical analgesic used in the treatment of mild to moderate pain. It has anti-inflammatory and antipyretic properties and acts as an inhibitor of cyclooxygenase which results in the inhibition of the biosynthesis of prostaglandins. Aspirin also inhibits platelet aggregation and is used in the prevention of arterial and venous thrombosis. (From Martindale, The Extra Pharmacopoeia, 30th ed, p5)	Acetylsalicylic Acid,2-(Acetyloxy)benzoic Acid,Acetysal,Acylpyrin,Aloxiprimum,Colfarit,Dispril,Easprin,Ecotrin,Endosprin,Magnecyl,Micristin,Polopirin,Polopiryna,Solprin,Solupsan,Zorprin,Acid, Acetylsalicylic
D012964	Sodium	A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.	Sodium Ion Level,Sodium-23,Ion Level, Sodium,Level, Sodium Ion,Sodium 23