Biomaterial Database

Semen collection by electroejaculation in the domestic cat. 1978

C C Platz, and S W Seager

A procedure for electroejaculation of domestic cats was developed. The method involved anesthetization of adult male cats with ketamine HC1 (33 mg/kg) or CI 744 (5.5 mg/kg). Optimal results were obtained with a 120-V ac electric stimulator capable of a continuous range of 0 to 60 V and 0 to 1 A. Using a 1- by 12-cm rectal probe constructed of teflon and stainless steel electrodes, ejaculation was obtained with an electrical stimulus over a range of 2 to 8 V and 5 to 220 mA. Voltage and amperage were increased at specific intervals in order to compensate for refractory ejaculatory response due to continued nerve and muscle stimulation. The procedure provided a means of obtaining ejaculates from domestic cats for purposes of semen evaluation and artificial insemination.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D002415	Cats	The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801)	Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D004542	Ejaculation	The emission of SEMEN to the exterior, resulting from the contraction of muscles surrounding the male internal urogenital ducts.	Ejaculations
D004558	Electric Stimulation	Use of electric potential or currents to elicit biological responses.	Stimulation, Electric,Electrical Stimulation,Electric Stimulations,Electrical Stimulations,Stimulation, Electrical,Stimulations, Electric,Stimulations, Electrical
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012661	Semen	The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma.	Seminal Plasma,Plasma, Seminal
D013048	Specimen Handling	Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.	Specimen Collection,Collection, Specimen,Collections, Specimen,Handling, Specimen,Handlings, Specimen,Specimen Collections,Specimen Handlings
D013081	Sperm Motility	Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression.	Motilities, Sperm,Motility, Sperm,Sperm Motilities