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A combined gas chromatographic-mass spectrometric technique is described for the quantification of virazole in serum and urine. Proteins are removed by molecular filtration, lipids by extraction with dichloromethane and interfering endogenous constituents by acidic and basic ion-exchange resins. Virazole is quantified by monitoring the protonated molecular ions of the fully silylated derivatives of virazole (m/e 533) and the arabinose analog (internal standard) obtained by methane chemical ionization. The detection limit is 150 pg (0.6.10(-12) mole) of virazole injected. In serum 10 ng/ml (4.10(-8) mole) can be detected, 25 ng/ml quantified. In urine 0.5 microgram/ml can be quantified without preconcentration. Virazole was detected in serum for at least 96 h at the 70-ng/ml level.
J Roboz, and
R Suzuki
June 1977,
Biochemical pharmacology,
J Roboz, and
R Suzuki
August 1972,
Science (New York, N.Y.),
J Roboz, and
R Suzuki
April 1973,
Antimicrobial agents and chemotherapy,
J Roboz, and
R Suzuki
August 1976,
The Journal of general virology,
J Roboz, and
R Suzuki
April 1973,
Antimicrobial agents and chemotherapy,
J Roboz, and
R Suzuki
June 1976,
Journal of medicinal chemistry,
J Roboz, and
R Suzuki
April 1973,
Proceedings of the National Academy of Sciences of the United States of America,
J Roboz, and
R Suzuki
February 1973,
Antimicrobial agents and chemotherapy,
J Roboz, and
R Suzuki
March 1977,
Annals of the New York Academy of Sciences,
J Roboz, and
R Suzuki
May 1975,
Antimicrobial agents and chemotherapy,
