Biomaterial Database

Determination of radiochemical purity and stability of 99mTc-diethyl HIDA. 1981

V Jovanović, and D Konstantinovska, and T Memedović

Several chromatographic methods have been used for determining the radiochemical purity of 99m Tc-diethyl HIDA (Solco HIDA). Good separation of 99mTc-HIDA from 99mTc-hydrolyzate and nonreduced 99mTcO4- was obtained in a short time using Gelman ITLC (SA) and 2N NaC1 as the mobile phase. In vitro stability was followed by the same method. It was found that the presence of oxygen from air enhances the percentage of 99mTcO4- in the preparation. Formation of a chelate of 99mTc-HIDA with a high stability constant was established by using Sephadex G-25 filtration. The degree of binding of 99mTc-diethyl HIDA to blood plasma proteins, examined by the same method. In vivo stability was examined by analyzing bile and urine, which were found to contain 99mTc-HIDA chelate. Biodistribution of the preparation was also investigated in experimental animals.

UI	MeSH Term	Description	Entries
D007098	Imino Acids	Carboxylic acids that contain an imino group (C	Amino Acids, Secondary,Acids, Secondary Amino,Secondary Amino Acids,Acids, Imino
D007413	Intestinal Mucosa	Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.	Intestinal Epithelium,Intestinal Glands,Epithelium, Intestinal,Gland, Intestinal,Glands, Intestinal,Intestinal Gland,Mucosa, Intestinal
D007668	Kidney	Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.	Kidneys
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008809	Mice, Inbred C3H	An inbred strain of mouse that is used as a general purpose strain in a wide variety of RESEARCH areas including CANCER; INFECTIOUS DISEASES; sensorineural, and cardiovascular biology research.	Mice, C3H,Mouse, C3H,Mouse, Inbred C3H,C3H Mice,C3H Mice, Inbred,C3H Mouse,C3H Mouse, Inbred,Inbred C3H Mice,Inbred C3H Mouse
D011786	Quality Control	A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed)	Control, Quality,Controls, Quality,Quality Controls
D004355	Drug Stability	The chemical and physical integrity of a pharmaceutical product.	Drug Shelf Life,Drugs Shelf Lives,Shelf Life, Drugs,Drug Stabilities,Drugs Shelf Life,Drugs Shelf Live,Life, Drugs Shelf,Shelf Life, Drug,Shelf Live, Drugs,Shelf Lives, Drugs
D004356	Drug Storage	The process of keeping pharmaceutical products in an appropriate location.	Drug Storages,Storage, Drug,Storages, Drug
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013667	Technetium	The first artificially produced element and a radioactive fission product of URANIUM. Technetium has the atomic symbol Tc, and atomic number 43. All technetium isotopes are radioactive. Technetium 99m (m	Technetium 99m,99m, Technetium