Biomaterial Database

Cross-linking of intact erythrocyte membrane with a newly synthesized cleavable bifunctional reagent. 1981

S Sato, and M Nakao

In order to clarify the interaction of membrane proteins in intact cells (not ghosts), a new bifunctional cleavable reagent, maleimidomethyl-3-maleimido propionate (MMP), was synthesized. Human intact erythrocytes and ghosts were incubated with this reagent. Cross-linked products were analyzed by two-dimensional polyacrylamide gel electrophoresis with incubation of the first dimensional gel in 0.1 N NaOH for 15 min at room temperature before the second run. In the case of ghosts, newly appeared bands were tentatively identified as a spectrin dimer, a complex of band 2 with band 5 degrees, which runs very slightly more slowly than band 5 and is phosphorylated by 32P in intact cells, a complex of band 3 with band 5, a dimer of band 3 and a dimer of band 5 degrees. In the case of intact cells, the newly appeared bands were complexes of band 3 with bands 4.3, 4.5, 4.6, and 4.7 and band 5 dimer, in addition to those seen in the case of ghosts. The technique of membrane phosphorylation permitted the clearer identification of each band. This reagent is useful for studying the relationship among cell membrane proteins in intact erythrocytes.

UI	MeSH Term	Description	Entries
D008301	Maleimides	Derivatives of maleimide (the structural formula H2C2(CO)2NH) containing a pyrroledione ring where the hydrogen atom of the NH group is replaced with aliphatic or aromatic groups.
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D003432	Cross-Linking Reagents	Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.	Bifunctional Reagent,Bifunctional Reagents,Cross Linking Reagent,Crosslinking Reagent,Cross Linking Reagents,Crosslinking Reagents,Linking Reagent, Cross,Linking Reagents, Cross,Reagent, Bifunctional,Reagent, Cross Linking,Reagent, Crosslinking,Reagents, Bifunctional,Reagents, Cross Linking,Reagents, Cross-Linking,Reagents, Crosslinking
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004910	Erythrocyte Membrane	The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.	Erythrocyte Ghost,Red Cell Cytoskeleton,Red Cell Ghost,Erythrocyte Cytoskeleton,Cytoskeleton, Erythrocyte,Cytoskeleton, Red Cell,Erythrocyte Cytoskeletons,Erythrocyte Ghosts,Erythrocyte Membranes,Ghost, Erythrocyte,Ghost, Red Cell,Membrane, Erythrocyte,Red Cell Cytoskeletons,Red Cell Ghosts
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man