Development of functional motor innervation in supernumerary hindlimbs of the chick embryo. 1978

D G Morris

1. The development of functional motor innervation in supernumerary chick hindlimbs transplated to host embryos at stages 16--18, was studied throughout the embryonic period from stage 27 when peripheral synapses were first formed. 2. The innervation pattern, defined as the number and sequential order of spinal nerves innervating individual muscles, were determined for supernumerary hindlimbs innervated by thoracic, lumbosacral-thoracic, or wholly lumbosacral spinal cord segments. Spinal nerves were electrically stimulated and muscle contraction was scored visually or by tension measurement and compound action-potential recordings were made from muscle nerves. 3. There was no tendency for spinal nerves to grow to or to synapse with the muscles which they normally innervate. Rather, they formed functional connections with inappropriate muscles, which were maintained throughout the developmental period studied. The localization of the motoneuron cell bodies was confirmed with retrograde transport of horseradish peroxidase (HRP) and shown to be adjacent to the spinal nerves through which their axons exited. Motoneurons innervating specific muscles occupied a similar medial-to-lateral position in the cord in both control and supernumerary limbs. 4. In all supernumerary limbs a definite peripheral innervation pattern was formed, which approximated the normal pattern. Specifically, the relative craniocaudal position of motor columns innervating individual muscles was conserved, even though the actual spinal nerves innervating the transplant were different from animal to animal. In limbs reversed along the anterior-posterior axis, the innervation pattern was also reversed. 5. The results suggest that motoneurons have not been rigidly specified to innervate certain muscles at the time when the limb buds were transplanted. Further, the limb itself can apparently influence the innervation pattern in an important manner, possibly by specifying motoneurons or by subsequently directing axon outgrowth and synapse formation.

UI MeSH Term Description Entries
D009046 Motor Neurons Neurons which activate MUSCLE CELLS. Neurons, Motor,Alpha Motorneurons,Motoneurons,Motor Neurons, Alpha,Neurons, Alpha Motor,Alpha Motor Neuron,Alpha Motor Neurons,Alpha Motorneuron,Motoneuron,Motor Neuron,Motor Neuron, Alpha,Motorneuron, Alpha,Motorneurons, Alpha,Neuron, Alpha Motor,Neuron, Motor
D009132 Muscles Contractile tissue that produces movement in animals. Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D009928 Organ Specificity Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen. Tissue Specificity,Organ Specificities,Specificities, Organ,Specificities, Tissue,Specificity, Organ,Specificity, Tissue,Tissue Specificities
D002642 Chick Embryo The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching. Embryo, Chick,Chick Embryos,Embryos, Chick
D006614 Hindlimb Either of two extremities of four-footed non-primate land animals. It usually consists of a FEMUR; TIBIA; and FIBULA; tarsals; METATARSALS; and TOES. (From Storer et al., General Zoology, 6th ed, p73) Hindlimbs
D000200 Action Potentials Abrupt changes in the membrane potential that sweep along the CELL MEMBRANE of excitable cells in response to excitation stimuli. Spike Potentials,Nerve Impulses,Action Potential,Impulse, Nerve,Impulses, Nerve,Nerve Impulse,Potential, Action,Potential, Spike,Potentials, Action,Potentials, Spike,Spike Potential
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013127 Spinal Nerves The 31 paired peripheral nerves formed by the union of the dorsal and ventral spinal roots from each spinal cord segment. The spinal nerve plexuses and the spinal roots are also included. Nerve, Spinal,Nerves, Spinal,Spinal Nerve
D014184 Transplantation, Homologous Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals. Transplantation, Allogeneic,Allogeneic Grafting,Allogeneic Transplantation,Allografting,Homografting,Homologous Transplantation,Grafting, Allogeneic

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