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Differential binding of IgG subclasses to enzyme-treated human lymphocytes. 1981

D Gilbert, and M Daveau, and M Fontaine

Human erythrocytes coated with each of the four human IgG subclasses were used to detect Fc gamma receptors on human peripheral blood lymphocytes (HPBL). Rosette formation was obtained only with erythrocytes coated with IgG3 (EA gamma 3). Neuraminidase treatment of HPBL induced rosette formation with EA gamma 1, EA gamma 2, and EA gamma 4 complexes. Pronase treatment also induced rosette formation to a lesser extent, but abolished EA gamma 3 rosetting. Trypsin treatment enhanced EA gamma 3 rosette formation. These phenomena occurred on both 'T' and 'non-T' lymphocytes. Erythrocytes coated with small quantities of IgG 3 did not form rosettes with HPBL. Neuraminidase treatment enhanced their binding, whereas pronase did not. These two phenomena occurred only on non-T lymphocytes. Rosette formation with EA gamma 1 was also obtained with lymphocytes stimulated in vitro with mitogens. After 2 days of culture, stimulated lymphocytes expressed receptors that were able to bind both EA gamma 1 and EA gamma 3 complexes. These results suggest the existence of cryptic receptors for IgG1, IgG2, and IgG4 that could be disclosed by neuraminidase and pronase treatment and exposed on stimulated lymphocytes. A hypothesis of one or several Fc gamma receptors is suggested.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007106 Immune Sera Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen. Antisera,Immune Serums,Sera, Immune,Serums, Immune
D008213 Lymphocyte Activation Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION. Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D008214 Lymphocytes White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS. Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D009194 Myeloma Proteins Abnormal immunoglobulins characteristic of MULTIPLE MYELOMA. M Components,Proteins, Myeloma
D009439 Neuraminidase An enzyme that catalyzes the hydrolysis of alpha-2,3, alpha-2,6-, and alpha-2,8-glycosidic linkages (at a decreasing rate, respectively) of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrate. (From Enzyme Nomenclature, 1992) Sialidase,Exo-alpha-Sialidase,N-Acylneuraminate Glycohydrolases,Oligosaccharide Sialidase,Exo alpha Sialidase,Glycohydrolases, N-Acylneuraminate,N Acylneuraminate Glycohydrolases,Sialidase, Oligosaccharide
D011402 Pronase A proteolytic enzyme obtained from Streptomyces griseus. Pronase E,Pronase P,Protease XIV,XIV, Protease
D011961 Receptors, Fc Molecules found on the surface of some, but not all, B-lymphocytes, T-lymphocytes, and macrophages, which recognize and combine with the Fc (crystallizable) portion of immunoglobulin molecules. Fc Receptors,Fc Receptor,Receptor, Fc
D004912 Erythrocytes Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN. Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

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