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A direct photometric method for assaying neurotoxic esterase (NTE) activity of chicken brain microsomal preparation has been developed using 4-nitrophenyl esters as substrates. Paired samples of the microsomal preparation were preincubated for 20 min with paraoxon plus either (a) buffer or (b) mipafox before addition of substrate. The initial rate of NTE activity was directly recorded at 410 nm by matching the content of tube (a) against tube (b) after addition of the substrate to both of them. The 4-nitrophenyl esters of propionic, butyric, valeric, lauric, capric and caproic acids were tested as substrates. Results indicated that 4-nitrophenyl valerate and caproate, respectively, are the most hydrolyzable substrates for NTE with this method; its also enables detailed kinetic studies of NTE to be made. The Michaelis constant (Km) for the hydrolysis of 4-nitrophenyl valerate by NTe was found to be 5.55 . 10(-5) M.
S A Soliman, and
A Curley, and
A K El-Sebae
January 1983,
Neurotoxicology,
S A Soliman, and
A Curley, and
A K El-Sebae
January 1991,
Fundamental and applied toxicology : official journal of the Society of Toxicology,
S A Soliman, and
A Curley, and
A K El-Sebae
January 1973,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
S A Soliman, and
A Curley, and
A K El-Sebae
March 1982,
Toxicology and applied pharmacology,
S A Soliman, and
A Curley, and
A K El-Sebae
June 1983,
Toxicology letters,
S A Soliman, and
A Curley, and
A K El-Sebae
February 1989,
Toxicology and applied pharmacology,
S A Soliman, and
A Curley, and
A K El-Sebae
June 1951,
The Journal of biological chemistry,
S A Soliman, and
A Curley, and
A K El-Sebae
June 1977,
Archives of toxicology,
S A Soliman, and
A Curley, and
A K El-Sebae
January 1985,
Toxicology and applied pharmacology,
S A Soliman, and
A Curley, and
A K El-Sebae
January 1989,
Biochemical pharmacology,
