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A gas chromatographic--mass fragmentographic method was developed for the determination of homopantothenic acid in plasma. Acidified plasma was deproteinized by extraction with chloroform and subsequently the aqueous layer was extracted with ethyl acetate. The organic layer containing homopantothenic acid was reduced to dryness, and the resulting residue was redissolved in N,O-bis(trimethylsilyl)trifluoroacetamide--pyridine solution to allow trimethylsilylation. Aliquots of this solution were injected into the gas chromatograph--mass spectrometer and analyzed by the selected ion monitoring method using L-ascorbic acid as an internal standard. The detection limit for homopantothenic acid was 5 ng/ml of plasma. A precise and sensitive assay for the determination of homopantothenic acid in plasma was established.
Y Umeno, and
K Nakai, and
E Matsushima, and
T Marunaka
October 1975,
Journal of chromatography,
Y Umeno, and
K Nakai, and
E Matsushima, and
T Marunaka
August 1975,
Clinica chimica acta; international journal of clinical chemistry,
Y Umeno, and
K Nakai, and
E Matsushima, and
T Marunaka
September 1987,
Journal of chromatography,
Y Umeno, and
K Nakai, and
E Matsushima, and
T Marunaka
August 1975,
Clinica chimica acta; international journal of clinical chemistry,
Y Umeno, and
K Nakai, and
E Matsushima, and
T Marunaka
December 1993,
Clinical biochemistry,
Y Umeno, and
K Nakai, and
E Matsushima, and
T Marunaka
March 1979,
Journal - Association of Official Analytical Chemists,
Y Umeno, and
K Nakai, and
E Matsushima, and
T Marunaka
September 1977,
Journal of pharmaceutical sciences,
Y Umeno, and
K Nakai, and
E Matsushima, and
T Marunaka
December 1977,
Journal of neurochemistry,
Y Umeno, and
K Nakai, and
E Matsushima, and
T Marunaka
May 1982,
Chemical & pharmaceutical bulletin,
Y Umeno, and
K Nakai, and
E Matsushima, and
T Marunaka
January 1977,
Acta pharmaceutica Suecica,
