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The hydrogen-bonded imino protons of a 14 base pair double-stranded DNA fragment comprising one half of the lac operator of E. coli were investigated by 360 MHz H NMR. From combined melting studies of this synthetic 14 b.p. fragment and its two constituent 7 b.p. fragments a nearly complete assignment for the low-field proton resonances was obtained. The experimental spectra are compared with calculated spectra and with the spectrum of a 51 b.p. DNA restriction fragment from E. coli containing the complete lac operator. Structural information on these oligonucleotides is presented. This study is a prerequisite for future 1H NMR investigations of the interaction of the lac operator with the lac repressor.
E R Zuiderweg, and
R M Scheek, and
G Veeneman, and
J H van Boom, and
R Kaptein, and
H Rüterjans, and
K Beyreuther
January 1982,
Proceedings of the National Academy of Sciences of the United States of America,
E R Zuiderweg, and
R M Scheek, and
G Veeneman, and
J H van Boom, and
R Kaptein, and
H Rüterjans, and
K Beyreuther
December 1967,
Proceedings of the National Academy of Sciences of the United States of America,
E R Zuiderweg, and
R M Scheek, and
G Veeneman, and
J H van Boom, and
R Kaptein, and
H Rüterjans, and
K Beyreuther
November 1984,
Journal of molecular biology,
E R Zuiderweg, and
R M Scheek, and
G Veeneman, and
J H van Boom, and
R Kaptein, and
H Rüterjans, and
K Beyreuther
January 1986,
Gene,
E R Zuiderweg, and
R M Scheek, and
G Veeneman, and
J H van Boom, and
R Kaptein, and
H Rüterjans, and
K Beyreuther
May 1983,
European journal of biochemistry,
E R Zuiderweg, and
R M Scheek, and
G Veeneman, and
J H van Boom, and
R Kaptein, and
H Rüterjans, and
K Beyreuther
July 1990,
Biochemical pharmacology,
E R Zuiderweg, and
R M Scheek, and
G Veeneman, and
J H van Boom, and
R Kaptein, and
H Rüterjans, and
K Beyreuther
September 1990,
Biochimica et biophysica acta,
E R Zuiderweg, and
R M Scheek, and
G Veeneman, and
J H van Boom, and
R Kaptein, and
H Rüterjans, and
K Beyreuther
June 1991,
Journal of molecular biology,
E R Zuiderweg, and
R M Scheek, and
G Veeneman, and
J H van Boom, and
R Kaptein, and
H Rüterjans, and
K Beyreuther
April 1986,
Journal of biomolecular structure & dynamics,
E R Zuiderweg, and
R M Scheek, and
G Veeneman, and
J H van Boom, and
R Kaptein, and
H Rüterjans, and
K Beyreuther
February 1970,
Journal of molecular biology,
