Biomaterial Database

Effect of vitamin E deficiency on the proliferative response of canine lymphocytes. 1981

M Langweiler, and R D Schultz, and B E Sheffy

The effect of vitamin E deficiency on the proliferative response of canine lymphocytes was examined, using the lymphocyte blastogenesis assay, with phytohemagglutinin, concanavalin A, and pokeweed as mitogenic stimulants. A litter (4 pups; group 3) from a bitch fed a complete diet was given a complete diet from the time of weaning (6 weeks) until termination of the experiment, and served as a control group. A litter (4 pups; group 1) from a bitch fed a vitamin E-deficient diet was given a vitamin E-deficient diet from the time of weaning (6 weeks) until termination of the experiment. A litter (6 pups; group 2) from a bitch fed a complete diet was weaned onto a complete diet and then changed to a deficient diet until termination of the experiment. Lymphocytes from group 1 and 2 pups (fed deficient diets) were poorly responsive to mitogen-induced blastogenesis, compared with the lymphocytic response seen in the controls. Washing the lymphocytes from the pups fed vitamin E-deficient diet resulted in enhanced responsiveness to mitogen stimulation. Lymphocytes from controls which had good responses when cultured in bovine fetal serum or normal canine serum were poorly responsive when cultured in serum from groups 1 and 2 pups (fed vitamin E-deficient diet). Reducing the concentration of group 1 and 2 pup sera in the culture medium restored the responsiveness of lymphocytes from control pups. Supplementation of group 1 and 2 pup sera with bovine fetal serum did not result in enhancement of the responses of lymphocytes from controls. The capability of sera from vitamin E-deficient pups (groups 1 and 2) to support blastogenesis of lymphocytes from control dogs was restored following dietary supplementation with vitamin E. It was concluded that the depressed lymphocyte responsiveness in pups fed vitamin E-deficient diet was due to the presense of a suppressive factor in the serum.

UI	MeSH Term	Description	Entries
D007106	Immune Sera	Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.	Antisera,Immune Serums,Sera, Immune,Serums, Immune
D008213	Lymphocyte Activation	Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.	Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D004283	Dog Diseases	Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.	Canine Diseases,Canine Disease,Disease, Canine,Disease, Dog,Diseases, Canine,Diseases, Dog,Dog Disease
D004285	Dogs	The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)	Canis familiaris,Dog
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006461	Hemolysis	The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.	Haemolysis,Extravascular Hemolysis,Intravascular Hemolysis,Extravascular Hemolyses,Haemolyses,Hemolyses, Extravascular,Hemolyses, Intravascular,Hemolysis, Extravascular,Hemolysis, Intravascular,Intravascular Hemolyses
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001463	Barbiturates	A class of chemicals derived from barbituric acid or thiobarbituric acid. Many of these are GABA MODULATORS used as HYPNOTICS AND SEDATIVES, as ANESTHETICS, or as ANTICONVULSANTS.
D014811	Vitamin E Deficiency	A nutritional condition produced by a deficiency of VITAMIN E in the diet, characterized by posterior column and spinocerebellar tract abnormalities, areflexia, ophthalmoplegia, and disturbances of gait, proprioception, and vibration. In premature infants vitamin E deficiency is associated with hemolytic anemia, thrombocytosis, edema, intraventricular hemorrhage, and increasing risk of retrolental fibroplasia and bronchopulmonary dysplasia. An apparent inborn error of vitamin E metabolism, named familial isolated vitamin E deficiency, has recently been identified. (Cecil Textbook of Medicine, 19th ed, p1181)	Deficiency, Vitamin E,Deficiencies, Vitamin E,Vitamin E Deficiencies