-transparent.png){kind=logo}
Sucrase--isomaltase was purified from rat intestinal microvillus membranes after injection of D-[2-3H]mannose and L-[6-3H]fucose, using a column of monoclonal antibody-protein A-Sepharose. After Pronase digestion and gel filtration of the glycopeptides labelled from both precursors, a major part of the radioactivity was recovered in asparagine-linked complex oligosaccharides, and a smaller amount in partially alkali-labile high-molecular-weight glycopeptides. Only a small amount of [3H]mannose was found in endo-beta-N-acetylglucosaminidase H-sensitive high-mannose oligosaccharides.
Related Publications
A Herscovics, and
A Quaroni, and
B Bugge, and
K Kirsch
September 1990,
Biochimica et biophysica acta,
A Herscovics, and
A Quaroni, and
B Bugge, and
K Kirsch
October 1975,
The New England journal of medicine,
A Herscovics, and
A Quaroni, and
B Bugge, and
K Kirsch
April 1982,
The Journal of biological chemistry,
A Herscovics, and
A Quaroni, and
B Bugge, and
K Kirsch
November 1982,
FEBS letters,
A Herscovics, and
A Quaroni, and
B Bugge, and
K Kirsch
January 1978,
Journal of nutritional science and vitaminology,
A Herscovics, and
A Quaroni, and
B Bugge, and
K Kirsch
September 1981,
Clinics in gastroenterology,
A Herscovics, and
A Quaroni, and
B Bugge, and
K Kirsch
April 1970,
The New England journal of medicine,
A Herscovics, and
A Quaroni, and
B Bugge, and
K Kirsch
July 2023,
The British journal of nutrition,
A Herscovics, and
A Quaroni, and
B Bugge, and
K Kirsch
September 1984,
European journal of biochemistry,
A Herscovics, and
A Quaroni, and
B Bugge, and
K Kirsch
May 1995,
Biochemical Society transactions,
