A simple strategy convenient for processing of RIA data and quality control in the laboratory on a desk-top-calculator. 1981

T Geier, and W Rohde

The experience with a computer strategy of mathematically simple models for the description of standard curves and continuous quality control calculations with outlier diagnostics for the processing of RIA data in laboratory is discussed. To get over the difficulties associated with the approximation of the nonlinear shape of standard curves, the combination of weighted iterative linear logit-log regression and point-to point logit-log interpolation is proposed. Monitoring of assay performance is based on three large pool sera determined in triplicates. The control data are checked by statistical tests for the rejection of single outlying observations, followed by calculation of objective criteria on within- and between-assay precision by means of analysis of variance for a one-way classification on the basis of which actual triplicates and whole assays can be assessed. Combined with quality control chart technique and the construction of a rough three-point precision profile the empirical knowledge of an experienced assayist should be included in the RIA interpretation as can be easily organized by way of a dialogue-program on a desk-top-calculator.

UI MeSH Term Description Entries
D011786 Quality Control A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed) Control, Quality,Controls, Quality,Quality Controls
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D003201 Computers Programmable electronic devices designed to accept data, perform prescribed mathematical and logical operations at high speed, and display the results of these operations. Calculators, Programmable,Computer Hardware,Computers, Digital,Hardware, Computer,Calculator, Programmable,Computer,Computer, Digital,Digital Computer,Digital Computers,Programmable Calculator,Programmable Calculators

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