-transparent.png){kind=logo}
The metabolism of 1,2-3H-androstenedione was studied in 2 cell lines, MCF-7 (estrogen responsive) and BT-20 (estrogen nonresponsive) over 48 hrs. Water soluble and unconjugated metabolites were separated by solvent partition and the former was submitted to chromatography on Sephadex LH-20 and enzyme hydrolysis. The resulting unconjugated steroids were separated by paper chromatography and identities were established by reverse isotope dilution. The unconjugated steroids initially obtained were separated by chromatography and identified by reverse isotope dilution. About 70% of the androstenedione was metabolized by both cell lines. However, the respective conversions to conjugates by MCF-7 and BT-20 were 31% and 0.32%. In the former, glucosiduronates predominated (94%) and consisted of androsterone (55%), etiocholanolone (9.4%) and androstanediol (5alpha-androstane-3alpha,17beta-diol) (9.3%). Androsterone comprised most of the unconjugated metabolites in both cell lines. Androstanediol was found in both cell lines, 2% in MCF-7 and 12% in BT-20. Testosterone, 5alpha-androstane-3,17-dione and 3beta-hydroxy-5alpha-androstan-17-one were isolated only from MCF-7. The metabolism of 3H-estriol was studied in a similar way. Both cell lines produced about equal amounts of estriol-3-sulfate (9%) and a compound with properties of estriol-3-glucosiduronate (0.15--0.5%). The results worthy of emphasis are: 1. The far greater conjugation of androgens exhibited by the MCF-7 cell lines as compared to the BT-20 cell lines; 2. In MCF-7, the high conversion of androstenedione to etiocholanolone (glucosiduronate form), a metabolite reported to form only in liver and sebaceous cysts; 3. The possible formation in both cell lines of estriol-3-glucosiduronate, normally a metabolite of the intestine.
U Raju, and
R J Sklarew, and
J Post, and
M Levitz
August 1978,
Cancer research,
U Raju, and
R J Sklarew, and
J Post, and
M Levitz
December 2015,
Hormone molecular biology and clinical investigation,
U Raju, and
R J Sklarew, and
J Post, and
M Levitz
March 2008,
Minerva endocrinologica,
U Raju, and
R J Sklarew, and
J Post, and
M Levitz
July 1955,
Proceedings of the Royal Society of Medicine,
U Raju, and
R J Sklarew, and
J Post, and
M Levitz
January 2018,
Frontiers in molecular biosciences,
U Raju, and
R J Sklarew, and
J Post, and
M Levitz
June 2004,
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
U Raju, and
R J Sklarew, and
J Post, and
M Levitz
January 1998,
Anticancer research,
U Raju, and
R J Sklarew, and
J Post, and
M Levitz
January 2013,
PloS one,
U Raju, and
R J Sklarew, and
J Post, and
M Levitz
December 1994,
Digestive diseases and sciences,
U Raju, and
R J Sklarew, and
J Post, and
M Levitz
December 1998,
The American journal of clinical nutrition,
