-transparent.png){kind=logo}
We describe a mechanized method for determining total estrogens in plasma during the last trimester of pregnancy. This method involves rapid enzymic hydrolysis, automatic extraction (in a tube) with cyclohexane/ethyl acetate, purification of the extracted material by anion-exchange chromatography on a disposable "mini-column" of Dowex AG1 X 2 (acetate form), and continuous-flow fluorometric quantification. Conditions of hydrolysis and extraction were studied. The mean within-day imprecision (CV) was 7% and between-day imprecision was 8.1%. Sensitivity was 75 nmol/L of plasma. Results are obtained in 4 h. The technique is quite suitable for routine determinations: 90 assays can be done by a team of three technicians in one working day.
C Goutte-Coussieu, and
D Sanchez, and
G Adessi, and
M F Jayle
October 1977,
Clinical chemistry,
C Goutte-Coussieu, and
D Sanchez, and
G Adessi, and
M F Jayle
April 1978,
Journal of endocrinological investigation,
C Goutte-Coussieu, and
D Sanchez, and
G Adessi, and
M F Jayle
January 1961,
Bulletin de la Federation des societes de gynecologie et dobstetrique de langue francaise,
C Goutte-Coussieu, and
D Sanchez, and
G Adessi, and
M F Jayle
November 1960,
Gynaecologia. International monthly review of obstetrics and gynecology. Revue internationale mensuelle d'obstetrique et de gynecologie. Monatsschrift fur Geburtshilfe und Gynakologie,
C Goutte-Coussieu, and
D Sanchez, and
G Adessi, and
M F Jayle
October 1977,
Obstetrics and gynecology,
C Goutte-Coussieu, and
D Sanchez, and
G Adessi, and
M F Jayle
April 1970,
The Journal of clinical endocrinology and metabolism,
C Goutte-Coussieu, and
D Sanchez, and
G Adessi, and
M F Jayle
January 1963,
Archiv fur Gynakologie,
C Goutte-Coussieu, and
D Sanchez, and
G Adessi, and
M F Jayle
March 1970,
Clinical chemistry,
C Goutte-Coussieu, and
D Sanchez, and
G Adessi, and
M F Jayle
February 1969,
Clinical chemistry,
C Goutte-Coussieu, and
D Sanchez, and
G Adessi, and
M F Jayle
August 1972,
The Journal of endocrinology,
