Biomaterial Database

Decreased phytohemagglutinin-induced aggregation and C5a-induced chemotaxis of human newborn neutrophils. 1980

A D Mease, and G W Fischer, and K W Hunter, and F B Ruymann

Phytohemmagglutinin (PHA)-induced lectin aggregation, chemotactic response to C5a, and random migration were measured on paired samples of neutrophils obtained from human peripheral blood and cord blood of normal newborn infants. The mean aggregation rate (+/- 1 SD) of adult neutrophils with PHA was 16.8 +/- 4.4 vs. 12.0 +/- 3.6 for newborn neutrophils (P less than 0.005), and the mean percent aggregation of adult neutrophils was 56.2 +/- 9.2 vs. 45.6 +/- 8.3 for newborn neutrophils (P less than 0.005). Exposure to newborn plasma had no affect on adult neutrophil aggregation. Whereas vinblastine (VBL) decreased both the percent and rate of PHA-induced adult neutrophil aggregation, only the rate of newborn neutrophil aggregation was reduced by VBL. Newborn neutrophil chemotactic response to C5a, was reduced by 80% (P less than 0.0025), and showed a positive correlation with percent PHA-induced aggregation (r = 0.6037, P less than 0.05). On the other hand, random migration was not significantly reduced and did not correlate with PHA-induced aggregation. These observations suggest that the decreased chemotactic responsiveness of newborn neutrophils may be due to developmental membrane differences which adversely affect the number and/or availability of C5a resceptors. Lectin-induced aggregation studies of other chemotactic defects may identify similar differences.

UI	MeSH Term	Description	Entries
D007231	Infant, Newborn	An infant during the first 28 days after birth.	Neonate,Newborns,Infants, Newborn,Neonates,Newborn,Newborn Infant,Newborn Infants
D009504	Neutrophils	Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.	LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D002449	Cell Aggregation	The phenomenon by which dissociated cells intermixed in vitro tend to group themselves with cells of their own type.	Aggregation, Cell,Aggregations, Cell,Cell Aggregations
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D002634	Chemotaxis, Leukocyte	The movement of leukocytes in response to a chemical concentration gradient or to products formed in an immunologic reaction.	Leukotaxis,Leukocyte Chemotaxis
D003182	Complement C5	C5 plays a central role in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C5 is cleaved by C5 CONVERTASE into COMPLEMENT C5A and COMPLEMENT C5B. The smaller fragment C5a is an ANAPHYLATOXIN and mediator of inflammatory process. The major fragment C5b binds to the membrane initiating the spontaneous assembly of the late complement components, C5-C9, into the MEMBRANE ATTACK COMPLEX.	C5 Complement,Complement 5,Complement C5, Precursor,Complement Component 5,Precursor C5,Pro-C5,Pro-complement 5,C5, Complement,C5, Precursor,C5, Precursor Complement,Complement, C5,Component 5, Complement,Precursor Complement C5,Pro C5,Pro complement 5
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D037102	Lectins	Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.	Animal Lectin,Animal Lectins,Isolectins,Lectin,Isolectin,Lectin, Animal,Lectins, Animal