Comparison of selective culture and serologic agglutination of Treponema hyodysenteriae for diagnosis of swine dysentery. 1980

L A Joens, and D L Harris

Samples of faeces and serum were collected from pigs of various ages on 21 farms. Faecal samples were cultured on trypticase soy agar containing 5% citrated bovine blood and 400 microgram per ml spectinomycin, incubated at 42 degrees C in Gaspak jars under an atmosphere of 80% hydrogen: 20% carbon dioxide. Antibody titres to Treponema hyodysenteriae were determined by a microtitration agglutination method using merthiolate-inactivated whole cell antigen prepared from a beta- haemolytic isolate. Results indicated that mean titres in pigs from which beta- haemolytic T hyodysenteriae was isolated were significantly higher than in pigs which yielded isolates of weak beta- haemolytic T innocens or in culturally negative pigs (P less than 0.0225). Mean titres of herds where beta- haemolytic T hyodysenteriae was isolated were significantly higher (P less than 0.005) than the mean titres of either of the other two groups. However, mean titres of herds where no isolates were obtained were not significantly different from mean titres of herds where weak beta- haemolytic T innocens was isolated.

UI MeSH Term Description Entries
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D004403 Dysentery Acute inflammation of the intestine associated with infectious DIARRHEA of various etiologies, generally acquired by eating contaminated food containing TOXINS, BIOLOGICAL derived from BACTERIA or other microorganisms. Dysentery is characterized initially by watery FECES then by bloody mucoid stools. It is often associated with ABDOMINAL PAIN; FEVER; and DEHYDRATION. Infectious Diarrheal Disease,Diarrheal Disease, Infectious,Infectious Diarrheal Diseases
D005243 Feces Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D000372 Agglutination Tests Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed) Agglutination Test,Test, Agglutination,Tests, Agglutination
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000907 Antibodies, Bacterial Immunoglobulins produced in a response to BACTERIAL ANTIGENS. Bacterial Antibodies
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D013553 Swine Diseases Diseases of domestic swine and of the wild boar of the genus Sus. Disease, Swine,Diseases, Swine,Swine Disease
D014208 Treponema A genus of microorganisms of the order SPIROCHAETALES, many of which are pathogenic and parasitic for man and animals.
D014211 Treponemal Infections Infections with bacteria of the genus TREPONEMA. Bejel,Infections, Treponemal,Bejels,Infection, Treponemal,Treponemal Infection

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