Biomaterial Database

Effect of genetic diabetes on enzymes of mouse intestinal brush-border membrane. 1980

K Ramaswamy, and P W Flint

Activities of intestinal enzymes were measured in genetically diabetic mouse of strain C57BL/KsJ-dbm to determine the long-term effects of genetic and uncontrolled diabetes on intestinal digestive function. Specific activities of enzymes were measured in intestinal homogenates, brush-border membrane fractions, and everted sacs from diabetic mice and their littermate controls. Sucrase, maltase, trehalase, alkaline phosphatase, and leucylnaphthylamidase activities were elevated in diabetes; lactase did not show any changes. The increases in disaccharidase activities in diabetes were in homogenates from both proximal and distal intestine but the increases in distal were more pronounced than in the proximal. Measurement of enzyme activities in brush-border membrane fractions showed a pattern similar to that observed in homogenates. Hydrolysis of sucrose and trehalose by everted sacs was markedly higher in the diabetic mice. It is therefore concluded that in genetic diabetes the digestive function of the intestine is stimulated, that the increased enzymes were incorporated into the brush-border membrane, and that the additional enzymes are accessible to the substrates in the intestinal lumen and so of physiological significance.

UI	MeSH Term	Description	Entries
D007421	Intestine, Small	The portion of the GASTROINTESTINAL TRACT between the PYLORUS of the STOMACH and the ILEOCECAL VALVE of the LARGE INTESTINE. It is divisible into three portions: the DUODENUM, the JEJUNUM, and the ILEUM.	Small Intestine,Intestines, Small,Small Intestines
D007931	Leucyl Aminopeptidase	A zinc containing enzyme of the hydrolase class that catalyzes the removal of the N-terminal amino acid from most L-peptides, particularly those with N-terminal leucine residues but not those with N-terminal lysine or arginine residues. This occurs in tissue cell cytosol, with high activity in the duodenum, liver, and kidney. The activity of this enzyme is commonly assayed using a leucine arylamide chromogenic substrate such as leucyl beta-naphthylamide.	Cytosol Aminopeptidase,Leucine Aminopeptidase,L-Leucylnaphthylamidase,Methoxyleucine Aminopeptidase,Peptidase S,Zinc-Manganese-Leucine Aminopeptidase,Aminopeptidase, Cytosol,Aminopeptidase, Leucine,Aminopeptidase, Leucyl,Aminopeptidase, Methoxyleucine,Aminopeptidase, Zinc-Manganese-Leucine,Zinc Manganese Leucine Aminopeptidase
D008297	Male		Males
D008810	Mice, Inbred C57BL	One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases.	Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008817	Mice, Mutant Strains	Mice bearing mutant genes which are phenotypically expressed in the animals.	Mouse, Mutant Strain,Mutant Mouse Strain,Mutant Strain of Mouse,Mutant Strains of Mice,Mice Mutant Strain,Mice Mutant Strains,Mouse Mutant Strain,Mouse Mutant Strains,Mouse Strain, Mutant,Mouse Strains, Mutant,Mutant Mouse Strains,Mutant Strain Mouse,Mutant Strains Mice,Strain Mouse, Mutant,Strain, Mutant Mouse,Strains Mice, Mutant,Strains, Mutant Mouse
D008871	Microvilli	Minute projections of cell membranes which greatly increase the surface area of the cell.	Brush Border,Striated Border,Border, Brush,Border, Striated,Borders, Brush,Borders, Striated,Brush Borders,Microvillus,Striated Borders
D003920	Diabetes Mellitus	A heterogeneous group of disorders characterized by HYPERGLYCEMIA and GLUCOSE INTOLERANCE.
D004063	Digestion	The process of breakdown of food for metabolism and use by the body.
D004186	Disaccharidases	Enzymes belonging to the class of GLYCOSIDE HYDROLASES which break down DISACCHARIDES into simpler sugars, MONOSACCHARIDES.	Disaccharidase
D005260	Female		Females