Enterotoxigenic staphylococci were isolated from cecum and rectum from 26 (5.8%) of 451 dogs. Of the 30 enterotoxigenic staphylococci isolated, 14 (46.7%) belonged to biotype E, which has been considered to be of canine origin (Baird-Parker's classification modified by Hájek and Marsálek). They produced exclusively enterotoxin type C. This is the first evidence of enterotoxigenic staphylococci of canine origin.