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A new spectrophotometric method for the determination of adenosine deaminase is described. Adenosine is deaminated to inosine, the latter is cleaved by an inosine-guanosine specific nucleoside phosphorylase to hypoxanthine and ribose-1-phosphate. Hypoxanthine can be oxidized further to uric acid by xanthine oxidase or to allantoin by xanthine oxidase and uricase. The hydrogen peroxide formed in these reactions is reduced by catalase to water. In the presence of high concentrations of ethanol, equivalent amounts of acetaldehyde are produced. The acetaldehyde is oxidized NAD(P) dependent and the production rate of NAD(P)H is recorded at 334 nm. The new method is suitable for the detection of adenosine deaminase in whole blood, lymphocytes, sera and tissues.
F Heinz, and
S Reckel, and
R Pilz, and
J R Kalden
January 1978,
Ciba Foundation symposium,
F Heinz, and
S Reckel, and
R Pilz, and
J R Kalden
August 1976,
Proceedings of the National Academy of Sciences of the United States of America,
F Heinz, and
S Reckel, and
R Pilz, and
J R Kalden
May 1982,
Analytical biochemistry,
F Heinz, and
S Reckel, and
R Pilz, and
J R Kalden
February 1983,
Analytical biochemistry,
F Heinz, and
S Reckel, and
R Pilz, and
J R Kalden
January 2012,
Frontiers in immunology,
F Heinz, and
S Reckel, and
R Pilz, and
J R Kalden
December 1988,
Clinica chimica acta; international journal of clinical chemistry,
F Heinz, and
S Reckel, and
R Pilz, and
J R Kalden
May 1977,
The Journal of laboratory and clinical medicine,
F Heinz, and
S Reckel, and
R Pilz, and
J R Kalden
April 1990,
Bollettino della Societa italiana di biologia sperimentale,
F Heinz, and
S Reckel, and
R Pilz, and
J R Kalden
February 1981,
Chemical & pharmaceutical bulletin,
F Heinz, and
S Reckel, and
R Pilz, and
J R Kalden
January 1985,
Annals of the New York Academy of Sciences,
