Biomaterial Database

Maturation and nucleo-cytoplasmic transport of rat-liver ribosomal RNA upon D-galactosamine inhibition of transcription. 1980

K C Gajdardjieva, and M D Dabeva, and A A Hadjiolov

D-Galactosamine (250 mg/kg body weight) causes 90--95% inhibition of [14C]orotate or inorganic [32P]phosphate incorporation in vivo into rat liver nuclear RNA within 30 min. The transcription of both nucleolar and nucleoplasmic genes is inhibited to the same extent. Under these conditions, prelabbeled 45-S pre-rRNA is processed quantitatively to nuclear 28-S and 18-S rRNA. The nucleocytoplasmic transport of both 28-S and 18-S rRNA remains unaltered for about 60 min after blockage of transcription. At this stage the nucleo-cytoplasmic transport of 18-S rRNA is almost completed. It is concluded that formation and nucleo-cytoplasmic transport of ribosomes is independent of concurrent transcription of rRNA or nucleoplasmic genes. At later stages, the nucleocytoplasmic transport of 28-S rRNA is delayed and its partial degaradation in the nucleus may take place. This effect is correlated with a decreased (up to 40% of controls) labelling of nuclear proteins. However, the labelling of total cellular or microsomal proteins remains unchanged up to 3 h after D-galactosamine administration. It is suggested that the last nuclear steps of ribosome formation are dependent on the continuous supply of rapidly-labelled nuclear proteins.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D002458	Cell Fractionation	Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.	Cell Fractionations,Fractionation, Cell,Fractionations, Cell
D002467	Cell Nucleus	Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D005260	Female		Females
D005688	Galactosamine
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001692	Biological Transport	The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.	Transport, Biological,Biologic Transport,Transport, Biologic
D012270	Ribosomes	Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION.	Ribosome
D012332	RNA, Heterogeneous Nuclear	Nuclear nonribosomal RNA larger than about 1000 nucleotides, the mass of which is rapidly synthesized and degraded within the cell nucleus. Some heterogeneous nuclear RNA may be a precursor to mRNA. However, the great bulk of total hnRNA hybridizes with nuclear DNA rather than with mRNA.	Heterogeneous Nuclear RNA,hnRNA,Nuclear RNA, Heterogeneous