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A subcutaneous (S.C.) was compared with an intradermal (I.D.) inoculation route of C-1300 neuroblastoma cells in adult A/J mice. This study was prompted by a recent report of increased metastases in intradermally inoculated murine neuroblastoma. The median survival in our line was longer in the I.D. group (44 days as compared to 34.5 days). Despite this feature, liver metastases, when detecp). In this series of experiments no other metastatic sites were found. Histologically both groups showed a similar picture under light microscopy, i.e., a highly undifferentiated tumor with little matrix and an absence of neuronal elements. It appears that the C-1300 neuroblastoma cell line utilized in this study differs biologically from that used by other investigators.
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