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Continuous-flow leukapheresis using the AMINCO Celltrifuge was performed in 35 adult volunteers for the procurement of large quantities of granulocyte/macrophage progenitor cells (CFUc) as part of the mononuclear leucocytes. The donor blood volume processed was 12 1 at a flow rate of 50 ml per min. The centrifuge speed was 800 rpm. The mean yield of mononuclear cells (MNC) per run was 12.0 x 10(9) and that of CFUc, 8.7 x 10(5). The leukapheresis did not much affect the donor blood cell concentration except the platelet concentration; this must be considered as a limiting factor for leukapheresis. The mean granulocyte contamination of the leukapheresis-derived leucocytes was only about 10%. Successive leukaphereses (with short-term intervals) in 7 donors led to significant increases in CFUc yield. The possible therapeutic application of blood stem cells in the treatment of haemopoietic failure in man is discussed.
M Körbling, and
T M Fliedner, and
H Pflieger
April 1972,
Blood,
M Körbling, and
T M Fliedner, and
H Pflieger
January 1984,
Journal of immunological methods,
M Körbling, and
T M Fliedner, and
H Pflieger
July 1974,
The New England journal of medicine,
M Körbling, and
T M Fliedner, and
H Pflieger
January 1975,
Transfusion,
M Körbling, and
T M Fliedner, and
H Pflieger
February 1976,
Blut,
M Körbling, and
T M Fliedner, and
H Pflieger
June 1995,
Transfusion,
M Körbling, and
T M Fliedner, and
H Pflieger
December 1979,
Revue francaise de transfusion et immuno-hematologie,
M Körbling, and
T M Fliedner, and
H Pflieger
January 1997,
Journal of clinical apheresis,
M Körbling, and
T M Fliedner, and
H Pflieger
September 1978,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
M Körbling, and
T M Fliedner, and
H Pflieger
January 1979,
Transfusion,
