-transparent.png){kind=logo}
We describe a new method for determining the biological activity of heparin in plasma with use of thrombin and the substrate Tos-Gly-Pro-Arg-pNA. The procedure is based on the photometric determination of the inactivation of thrombin after incubation with plasma in the presence of endogenous antithrombin III (At III). The method allows the specific determination of heparin concentrations from 0.02 USP to 0.8 USP/ml of plasma in the presence of normal At III levels. It has been carried out manually by use of an Eppendorf spectrum line photometer or automatically by use of a Vitatron Akes analyzer. For evaluation, the results were compared with two standard samples which contained heparin in the low and high therapeutic range, respectively.
K Bartl, and
E Dorsch, and
H Lill, and
J Ziegenhorn
April 1987,
Clinical chemistry,
K Bartl, and
E Dorsch, and
H Lill, and
J Ziegenhorn
January 1982,
Acta chirurgica Scandinavica. Supplementum,
K Bartl, and
E Dorsch, and
H Lill, and
J Ziegenhorn
May 1987,
Rinsho byori. The Japanese journal of clinical pathology,
K Bartl, and
E Dorsch, and
H Lill, and
J Ziegenhorn
September 1985,
Quaderni Sclavo di diagnostica clinica e di laboratorio,
K Bartl, and
E Dorsch, and
H Lill, and
J Ziegenhorn
January 1973,
Toxicon : official journal of the International Society on Toxinology,
K Bartl, and
E Dorsch, and
H Lill, and
J Ziegenhorn
January 1991,
Laboratornoe delo,
K Bartl, and
E Dorsch, and
H Lill, and
J Ziegenhorn
March 1981,
Thrombosis research,
K Bartl, and
E Dorsch, and
H Lill, and
J Ziegenhorn
December 1976,
Thrombosis and haemostasis,
K Bartl, and
E Dorsch, and
H Lill, and
J Ziegenhorn
January 1970,
Clinical chemistry,
K Bartl, and
E Dorsch, and
H Lill, and
J Ziegenhorn
December 1978,
Thrombosis research,
