BIOMAT Database Logo BIOMAT Database Logo

Genetic variants involving the major membrane sialoglycoprotein of human erythrocytes. Studies on erythrocytes of type Mk, Miltenberger class V and Mg. 1978

D J Anstee, and M J Tanner

1. Membranes from erythrocytes heterozygous for the Mk and Miltenberger Class V (Mi.V) condition and membranes from erythrocytes homozygous for the Mg condition were studied by polyacrylamide-gel electrophoresis by using the periodate/Schiff stain binding of radioiodinated lectins and labelling with lactoperoxidase. 2. Both the Mk and Mi.V conditions are associated with a decreased content of the major blood-group-MN-active sialoglycoprotein. 3. An unusual blood-group-M-active membrane component was found in Mi.V cells of appropriate genotype. No comparably component was found in Mk erythrocytes. 4. The Mg antigen appears to result from a modification of the MN-active sialoglycoprotein found in normal cells. Our results suggest that the Mg sialoglycoprotein contains fewer sialotetrasaccharides than does the normal sialglycoprotein. This may result from changes in the amino acid sequence of the protein. 5. The results are discussed in relation to differences in the antigenic properties of Mk, Mi.V and Mg cells and their possible influence on the structure of the surface of each of these cells.

UI MeSH Term Description Entries
D008951 MNSs Blood-Group System A system of universal human blood group isoantigens with many associated subgroups. The M and N traits are codominant and the S and s traits are probably very closely linked alleles, including the U antigen. This system is most frequently used in paternity studies. Blood-Group System, MNSs,Blood-Group Systems, MNSs,MNSs Blood Group System,MNSs Blood-Group Systems,System, MNSs Blood-Group,Systems, MNSs Blood-Group
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004910 Erythrocyte Membrane The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS. Erythrocyte Ghost,Red Cell Cytoskeleton,Red Cell Ghost,Erythrocyte Cytoskeleton,Cytoskeleton, Erythrocyte,Cytoskeleton, Red Cell,Erythrocyte Cytoskeletons,Erythrocyte Ghosts,Erythrocyte Membranes,Ghost, Erythrocyte,Ghost, Red Cell,Membrane, Erythrocyte,Red Cell Cytoskeletons,Red Cell Ghosts
D004912 Erythrocytes Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN. Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000941 Antigens Substances that are recognized by the immune system and induce an immune reaction. Antigen
D012795 Sialoglycoproteins Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities. Polysialoglycoprotein,Sialoglycopeptide,Sialoglycopeptides,Sialoglycoprotein,Sialoprotein,Sialoproteins,Polysialoglycoproteins

Related Publications

D J Anstee, and M J Tanner
Structural analysis of the major human erythrocyte membrane sialoglycoprotein from Miltenberger class VII cells.
July 1987, European journal of biochemistry,
D J Anstee, and M J Tanner
Studies on Miltenberger class III, V, Mv and Mk red cells. I. Sodium-dodecylsulfate polyacrylamide gel electrophoretic investigations.
September 1978, Blut,
D J Anstee, and M J Tanner
Structures of Miltenberger class I and II specific major human erythrocyte membrane sialoglycoproteins.
January 1984, European journal of biochemistry,
D J Anstee, and M J Tanner
Amino acid sequence of the blood group Mg-specific major human erythrocyte membrane sialoglycoprotein.
January 1981, Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
D J Anstee, and M J Tanner
Molecular analysis of glycophorin A and B gene structure and expression in homozygous Miltenberger class V (Mi. V) human erythrocytes.
September 1989, European journal of biochemistry,
D J Anstee, and M J Tanner
Membrane sialoglycoprotein from human erythrocytes activates lysosomal proteinases.
January 1979, Acta biologica et medica Germanica,
D J Anstee, and M J Tanner
Studies on the major sialoglycoprotein of the human red cell membrane. Isolation and characterization of tryptic glycopeptides.
July 1973, Biochemistry,
D J Anstee, and M J Tanner
Physiologically instructive genetic variants involving the human red cell membrane.
January 1983, Physiological reviews,
D J Anstee, and M J Tanner
Absence of the major sialoglycoprotein in the membrane of human En(a--) erythrocytes and increased glycosylation of band 3.
October 1976, The Journal of biological chemistry,
D J Anstee, and M J Tanner
Structural analysis of glycophorin A from Miltenberger class VIII erythrocytes.
August 1989, Biological chemistry Hoppe-Seyler,
Copied contents to your clipboard!