Biomaterial Database

Molarity and ionic strength of focused carrier ampholytes in isoelectric focusing. 1980

P G Righetti

Focused 1% carrier ampholytes in the pH range 3.5--10 have a molarity of 9--10 mM, as determined by ismolarity measurements of fractions focused in free water. From electrophoretic and isoelectric focusing data of red blood cells, it has been demonstrated that the corresponding ionic strength is 0.5 mg-ions/l. Also, theoretical considerations and conductivity measurements point to a value in the range of 0.5--1.0 mg-ions/l. The following equations for ionic strength (I) calculations have been derived: 1 = 1/20 C amph + CH in the pH range 2.5--7 and I = 1/20 Camph + COH in the pH range 7--11.

UI	MeSH Term	Description	Entries
D007525	Isoelectric Focusing	Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.	Electrofocusing,Focusing, Isoelectric
D002021	Buffers	A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.	Buffer
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000665	Ampholyte Mixtures	Such mixtures of amphoteric electrolytes or buffers that provide a continuous range of pH in an electric field; used for separating proteins by their isoelectric points, i.e., by isoelectric focusing.	Ampholines,Ampholyte,Ampholyte Mixture,Carrier Ampholyte,Carrier Ampholytes,Isoelectric Focusing Agent,Isoelectric Focusing Agents,Pharmalyte,Pharmalytes,Ampholytes,Servalyte,Agent, Isoelectric Focusing,Ampholytes, Carrier