Biomaterial Database

17-Hydroxycorticosteroids in urine. 1980

M Simon, and M Babich-Armstrong

A new method has been devised to extract 17-hydroxycorticosteroids (17OH-CS) from a hydrolyzed urine using an EXTUBE fiber column. This extraction was combined with a modified Porter-Silber color reaction to quantitate total urinary 17OH-CS. The new method takes only half the time required in older methods. Recovery of tetrahydrocortisone in the new method was improved to 85--95% compared with 70% in the previous method. Between-run precision (C.V.%) was concentration dependent in both methods and was improved to 5--7% compared with 10--18% in the previous method. Evidence was obtained to demonstrate that the new methodology would provide the same information and interpretation as the older, more cumbersome method.

UI	MeSH Term	Description	Entries
D008752	Methylene Chloride	A chlorinated hydrocarbon that has been used as an inhalation anesthetic and acts as a narcotic in high concentrations. Its primary use is as a solvent in manufacturing and food technology.	Dichloromethane,Methylene Bichloride,Methylene Dichloride,Solaesthin,Bichloride, Methylene,Chloride, Methylene,Dichloride, Methylene
D002845	Chromatography	Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.	Chromatographies
D005069	Evaluation Studies as Topic	Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies.	Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015065	17-Hydroxycorticosteroids	A group of hydroxycorticosteroids bearing a hydroxy group at the 17-position. Urinary excretion of these compounds is used as an index of adrenal function. They are used systemically in the free alcohol form, but with esterification of the hydroxy groups, topical effectiveness is increased.	17 Hydroxycorticosteroids