Biomaterial Database

Effects of nonhistone proteins on the circular dichroism spectrum and transcriptional activity of a DNA-histone complex. 1980

T Suzuki, and R Goto, and O Tamemasa

Nonhistone chromasomal proteins (NHP) of Ehrlich ascites tumor cells were fractionated into four subfractions by successive extractions with 0.35 M, 0.45 M, and 0.60 M NaCl, and finally 2.0 M NaCl + 5.0 M urea. The CD spectra and transcriptional activities of complexes reconstituted from DNA, histone, and each of these four NHP subfractions obtained from Ehrlich tumor cells were measured in order to identify the effect of each NHP subfraction. The positive ellipticity in the CD of the DNA-histone complex was considerably increased when reconstituted with 0.35 M- or 0.45 M-NHP. The 0.35 M- and 0.45 M-NHP also increased the transcriptional activity of DNA-histone complex. The activities of DNA-histone-0.35 M NHP complex increased progressively with increase in the 0.35 M NHP/DNA ratio. These results suggest that there is a correlation between the increase in transcriptional activity of DNA-histone complexes with 0.35 M- or 0.45 M-NHP and the increase in the percentage of DNA in the B conformation in complexes.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D009690	Nucleic Acid Conformation	The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.	DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D009698	Nucleoproteins	Proteins conjugated with nucleic acids.	Nucleoprotein
D011487	Protein Conformation	The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).	Conformation, Protein,Conformations, Protein,Protein Conformations
D002286	Carcinoma, Ehrlich Tumor	A transplantable, poorly differentiated malignant tumor which appeared originally as a spontaneous breast carcinoma in a mouse. It grows in both solid and ascitic forms.	Ehrlich Ascites Tumor,Ascites Tumor, Ehrlich,Ehrlich Tumor Carcinoma,Tumor, Ehrlich Ascites
D002467	Cell Nucleus	Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D002843	Chromatin	The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.	Chromatins
D002868	Chromosomal Proteins, Non-Histone	Nucleoproteins, which in contrast to HISTONES, are acid insoluble. They are involved in chromosomal functions; e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens.	Non-Histone Chromosomal Proteins,Chromosomal Proteins, Non Histone,Chromosomal Proteins, Nonhistone,Non-Histone Chromosomal Phosphoproteins,Chromosomal Phosphoproteins, Non-Histone,Non Histone Chromosomal Phosphoproteins,Non Histone Chromosomal Proteins,Nonhistone Chromosomal Proteins,Proteins, Non-Histone Chromosomal
D002942	Circular Dichroism	A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Circular Dichroism, Vibrational,Dichroism, Circular,Vibrational Circular Dichroism
D003852	Deoxyribonucleoproteins	Proteins conjugated with deoxyribonucleic acids (DNA) or specific DNA.